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浏览/检索结果: 共1条，第1-1条 帮助 限定条件 专题：湖南农业大学    第一署名单位    第一作者单位    通讯作者单位     已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 Proteomic analysis of cold stress responses in tobacco seedlings 期刊论文 African Journal of Biotechnology, 2011, 卷号: 10, 期号: 82, 页码: 18991-19004 作者:  Jin, Yan;  Zhang, Caohao;  Yang, Hui;  Yang, Yuhong;  Huang, Chengjiang 收藏  |  浏览/下载：2/0  |  提交时间：2019/12/23 Cold stress is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. To gain a better understanding of cold stress responses in tobacco (Nicotiana tabacum)  we carried out a comparative proteomic analysis. Five-week-old tobacco seedlings were treated at 4°C for 4 h. Cold treatment resulted in stress phenotypes of smoothing and shallowing leaves and increased relative electrolyte leakage. The expression changes of total proteins in tobacco leaves were examined using two-dimensional electrophoresis. Quantitative image analysis revealed a total of 101 protein spots that changed their intensities significantly  21 protein spots were down-regulated  eight were up-regulated after the cold treatment  50 protein spots only expressed in the control sample  while 22 protein spots were only present in the cold treatment sample. Mass spectrometry analysis allowed the identification of 73 differentially expressed proteins  including well known and novel cold-responsive proteins. The identified proteins are involved in several processes such as photosynthesis  protein processing  redox homeostasis  ribonucleic acid (RNA) processing  signal transduction  translation  cell division/cycle  and metabolisms of carbon and energy. Several types of proteins showed enhanced degradation during chilling stress  especially the photosynthetic proteins. Gene expression analysis of 25 different proteins by reverse transcriptase- polymerase chain reaction (RT-PCR) showed that the messenger RNA (mRNA) levels of 18 genes correlated well with the protein levels. In conclusion  our study provides new insights into cold stress responses in tobacco and needs to be further studied in future.