QQ客服 官方微博 反馈留言

	Proteomic analysis of cold stress responses in tobacco seedlings
	Jin, Yan; Zhang, Caohao; Yang, Hui; Yang, Yuhong; Huang, Chengjiang; Tian, Yun*; Lu, Xiangyang
刊名	African Journal of Biotechnology
	2011
卷号	10期号:82页码:18991-19004
关键词	Cold stress is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. To gain a better understanding of cold stress responses in tobacco (Nicotiana tabacum) we carried out a comparative proteomic analysis. Five-week-old tobacco seedlings were treated at 4°C for 4 h. Cold treatment resulted in stress phenotypes of smoothing and shallowing leaves and increased relative electrolyte leakage. The expression changes of total proteins in tobacco leaves were examined using two-dimensional electrophoresis. Quantitative image analysis revealed a total of 101 protein spots that changed their intensities significantly 21 protein spots were down-regulated eight were up-regulated after the cold treatment 50 protein spots only expressed in the control sample while 22 protein spots were only present in the cold treatment sample. Mass spectrometry analysis allowed the identification of 73 differentially expressed proteins including well known and novel cold-responsive proteins. The identified proteins are involved in several processes such as photosynthesis protein processing redox homeostasis ribonucleic acid (RNA) processing signal transduction translation cell division/cycle and metabolisms of carbon and energy. Several types of proteins showed enhanced degradation during chilling stress especially the photosynthetic proteins. Gene expression analysis of 25 different proteins by reverse transcriptase- polymerase chain reaction (RT-PCR) showed that the messenger RNA (mRNA) levels of 18 genes correlated well with the protein levels. In conclusion our study provides new insights into cold stress responses in tobacco and needs to be further studied in future.
ISSN号	1684-5315
DOI	10.5897/AJB11.900
URL标识	查看原文
WOS记录号	WOS:000298560900009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/5141707
专题	湖南农业大学
作者单位	1.[Jin, Yan 2.Yang, Yuhong] Tobacco Sci Res Inst Yunnan Prov, Yuxi 653100, Peoples R China.
推荐引用方式 GB/T 7714	Jin, Yan,Zhang, Caohao,Yang, Hui,et al. Proteomic analysis of cold stress responses in tobacco seedlings[J]. African Journal of Biotechnology,2011,10(82):18991-19004.
APA	Jin, Yan.,Zhang, Caohao.,Yang, Hui.,Yang, Yuhong.,Huang, Chengjiang.,...&Lu, Xiangyang.(2011).Proteomic analysis of cold stress responses in tobacco seedlings.African Journal of Biotechnology,10(82),18991-19004.
MLA	Jin, Yan,et al."Proteomic analysis of cold stress responses in tobacco seedlings".African Journal of Biotechnology 10.82(2011):18991-19004.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。