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Natural variation in the HAN1 gene confers chilling tolerance in rice and allowed adaptation to a temperate climate 期刊论文
Proceedings of the National Academy of Sciences of the United States of America, 2019, 卷号: 116, 期号: 9, 页码: 3494-3501
作者:  Mao, Donghai;  Xin, Yeyun;  Tan, Yongjun;  Hu, Xiaojie;  Bai, Jiaojiao
收藏  |  浏览/下载:68/0  |  提交时间:2019/12/23
Comparative Transcriptome Analyses Revealed Conserved and Novel Responses to Cold and Freezing Stress in Brassica napus L. 期刊论文
G3 (Bethesda, Md.), 2019, 卷号: 9, 期号: 8, 页码: 2723-2737
作者:  Xin, He;  Ni Xianchao;  Pan, Xie;  Wei, Liu;  Min, Yao
收藏  |  浏览/下载:5/0  |  提交时间:2019/12/23
Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum 期刊论文
Gene, 2012, 卷号: 501, 期号: 1, 页码: 52-62
作者:  Bai, Miao;  Yang, Guo-Shun;  Chen, Wen-Ting;  Mao, Zhen-Chuan;  Kang, Hou-Xiang
收藏  |  浏览/下载:1/0  |  提交时间:2019/12/23
AGO  Argonaute  CS  chilling stress  CDS  coding DNA sequence  CK  control  DCL  Dicer-like protein  dsRNAs  double-stranded RNAs  DS  drought stress  HS  heat stress  hc-siRNAs  heterochromatic siRNAs  ITAG  International Tomato Annotation Group  miRNAs  microRNAs  MS  Murashige and Skoog medium  OS  oxidative stress  piRNAs  PIWI-interacting RNAs  PTGS  post-transcriptional gene silencing  qPCR  Quantitative reverse transcription PCR  RT-PCR  reverse transcription PCR  RDR  RNA-dependent RNA polymerase  RdRP  RNA-dependent RNA polymerase domain  RISC  RNA-induced silencing complex  SA  salicylic acid  SS  salt stress  siRNAs  short-interfering RNAs  SMART  Simple Modular Architecture Research Tool  TYLCV  tomato yellow leaf curl virus  TYLCD  tomato yellow leaf curl disease  RNA silencing  MicroRNA  Phylogenetic analysis  Tomato yellow leaf curl virus  
Proteomic analysis of cold stress responses in tobacco seedlings 期刊论文
African Journal of Biotechnology, 2011, 卷号: 10, 期号: 82, 页码: 18991-19004
作者:  Jin, Yan;  Zhang, Caohao;  Yang, Hui;  Yang, Yuhong;  Huang, Chengjiang
收藏  |  浏览/下载:2/0  |  提交时间:2019/12/23
Cold stress is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. To gain a better understanding of cold stress responses in tobacco (Nicotiana tabacum)  we carried out a comparative proteomic analysis. Five-week-old tobacco seedlings were treated at 4°C for 4 h. Cold treatment resulted in stress phenotypes of smoothing and shallowing leaves and increased relative electrolyte leakage. The expression changes of total proteins in tobacco leaves were examined using two-dimensional electrophoresis. Quantitative image analysis revealed a total of 101 protein spots that changed their intensities significantly  21 protein spots were down-regulated  eight were up-regulated after the cold treatment  50 protein spots only expressed in the control sample  while 22 protein spots were only present in the cold treatment sample. Mass spectrometry analysis allowed the identification of 73 differentially expressed proteins  including well known and novel cold-responsive proteins. The identified proteins are involved in several processes such as photosynthesis  protein processing  redox homeostasis  ribonucleic acid (RNA) processing  signal transduction  translation  cell division/cycle  and metabolisms of carbon and energy. Several types of proteins showed enhanced degradation during chilling stress  especially the photosynthetic proteins. Gene expression analysis of 25 different proteins by reverse transcriptase- polymerase chain reaction (RT-PCR) showed that the messenger RNA (mRNA) levels of 18 genes correlated well with the protein levels. In conclusion  our study provides new insights into cold stress responses in tobacco and needs to be further studied in future.  


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