A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress

doi:10.1021/acs.jafc.9b04069

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	A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress
	Yang, Yunqiang1,2,3 ; Pu, Yanan 1,2,3; Yin, Xin 1,2,3,4; Du, Jiancan 2; Zhou, Zhili 1,2,3; Yang, Danni 1,2,3,4; Sun, Xudong 1,2,3; Sun, Hang1,2,3 ; Yang, Yongping1,2,3
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-10-09
卷号	67 期号:40 页码:11077-11088
关键词	wax esters drought stress alternative splicing turnip BrrWSD1
ISSN号	0021-8561
DOI	10.1021/acs.jafc.9b04069
通讯作者	Yang, Yongping(yangyp@mail.kib.ac.cn)
英文摘要	Cuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapa var. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a. BrrWSD1-X2 had a stronger ability to synthesize wax esters than BrrWSD1-X1 using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron of BrrWSD1 DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence of BrrWSD1-X2 in waxy varieties.
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
WOS记录号	WOS:000490354700009
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/69964]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Yang, Yongping
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yunqiang,Pu, Yanan,Yin, Xin,et al. A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(40):11077-11088.
APA	Yang, Yunqiang.,Pu, Yanan.,Yin, Xin.,Du, Jiancan.,Zhou, Zhili.,...&Yang, Yongping.(2019).A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(40),11077-11088.
MLA	Yang, Yunqiang,et al."A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.40(2019):11077-11088.