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	The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation
	Ma, Suofeng; Lin, Hang; Miao, Yiliang; Liu, Xinyong; Wang, Bin; Dai, Jianwu
刊名	Biomaterials
	2007-07-01
卷号	28期号:21页码:3198-3207
关键词	Co-culture Demineralized bone matrix In vitro maturation Oocyte
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2007.03.007
通讯作者	Dai, jianwu(jwdai@genetics.ac.cn)
英文摘要	The physiological role of cumulus cell surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. collagen-based demineralized bone matrix (dbm) is a valuable biomaterial for the three-dimensional (3-d) cell culture. the present study was designed to determine whether in vitro maturation (ivm) of cumulus-free oocytes in mice could be improved by using the 3-d dbm co-culture system. the results indicated that the denuded oocytes cultured in 3-d dbm co-culture system with cumulus cells showed close similarity of cortical granules (cgs) distribution pattern, had more normal maturation-promoting factor (mpf) level and zona pellucida (zp) hardening level to the in vivo matured oocytes, and the best preimplantation development after being activated by in vitro fertilization (ivf) or parthenogenetic activation. thus, 3-d dbm collagen scaffold could serve as a tool for fundamental in vitro studies of cells or tissues under the environment that closely assembles the in vivo conditions. (c) 2007 elsevier ltd. all rights reserved.
WOS关键词	MOUSE OOCYTES ; MEIOTIC MATURATION ; CORTICAL GRANULES ; ZONA-PELLUCIDA ; EXTRACELLULAR-MATRIX ; DEVELOPMENTAL COMPETENCE ; BOVINE OOCYTES ; SOMATIC-CELLS ; CULTURE ; FERTILIZATION
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000247056500004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379996
专题	中国科学院大学
通讯作者	Dai, Jianwu
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 4.Cent Hosp Taian, Med Ctr Reprod, Taian City, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ma, Suofeng,Lin, Hang,Miao, Yiliang,et al. The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation[J]. Biomaterials,2007,28(21):3198-3207.
APA	Ma, Suofeng,Lin, Hang,Miao, Yiliang,Liu, Xinyong,Wang, Bin,&Dai, Jianwu.(2007).The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation.Biomaterials,28(21),3198-3207.
MLA	Ma, Suofeng,et al."The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation".Biomaterials 28.21(2007):3198-3207.

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