The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation | |
Ma, Suofeng; Lin, Hang; Miao, Yiliang; Liu, Xinyong; Wang, Bin; Dai, Jianwu | |
刊名 | Biomaterials |
2007-07-01 | |
卷号 | 28期号:21页码:3198-3207 |
关键词 | Co-culture Demineralized bone matrix In vitro maturation Oocyte |
ISSN号 | 0142-9612 |
DOI | 10.1016/j.biomaterials.2007.03.007 |
通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
英文摘要 | The physiological role of cumulus cell surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. collagen-based demineralized bone matrix (dbm) is a valuable biomaterial for the three-dimensional (3-d) cell culture. the present study was designed to determine whether in vitro maturation (ivm) of cumulus-free oocytes in mice could be improved by using the 3-d dbm co-culture system. the results indicated that the denuded oocytes cultured in 3-d dbm co-culture system with cumulus cells showed close similarity of cortical granules (cgs) distribution pattern, had more normal maturation-promoting factor (mpf) level and zona pellucida (zp) hardening level to the in vivo matured oocytes, and the best preimplantation development after being activated by in vitro fertilization (ivf) or parthenogenetic activation. thus, 3-d dbm collagen scaffold could serve as a tool for fundamental in vitro studies of cells or tissues under the environment that closely assembles the in vivo conditions. (c) 2007 elsevier ltd. all rights reserved. |
WOS关键词 | MOUSE OOCYTES ; MEIOTIC MATURATION ; CORTICAL GRANULES ; ZONA-PELLUCIDA ; EXTRACELLULAR-MATRIX ; DEVELOPMENTAL COMPETENCE ; BOVINE OOCYTES ; SOMATIC-CELLS ; CULTURE ; FERTILIZATION |
WOS研究方向 | Engineering ; Materials Science |
WOS类目 | Engineering, Biomedical ; Materials Science, Biomaterials |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000247056500004 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2379996 |
专题 | 中国科学院大学 |
通讯作者 | Dai, Jianwu |
作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 4.Cent Hosp Taian, Med Ctr Reprod, Taian City, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Suofeng,Lin, Hang,Miao, Yiliang,et al. The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation[J]. Biomaterials,2007,28(21):3198-3207. |
APA | Ma, Suofeng,Lin, Hang,Miao, Yiliang,Liu, Xinyong,Wang, Bin,&Dai, Jianwu.(2007).The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation.Biomaterials,28(21),3198-3207. |
MLA | Ma, Suofeng,et al."The effect of three-dimensional demineralized bone matrix on in vitro cumulus-free oocyte maturation".Biomaterials 28.21(2007):3198-3207. |
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