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	Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph
	Dong, Sicong1,2,4; Wang, Manli1,2; Qiu, Zhijuan1,2; Deng, Fei1,2; Vlak, Just M.3; Hu, Zhihong1,2; Wang, Hualin1,2
刊名	Journal of virology
	2010-05-01
卷号	84期号:10页码:5351-5359
ISSN号	0022-538X
DOI	10.1128/jvi.02517-09
通讯作者	Wang, hualin(h.wang@wh.iov.cn)
英文摘要	The budded virus (bv) of the autographa californica multicapsid nucleopolyhedrovirus (acmnpv) infects insect cells and transduces mammalian cells mainly through the endocytosis pathway. however, this study revealed that the treatment of the virus bound to sf9 cells at low ph could efficiently rescue the infectivity of acmnpv in the presence of endocytosis pathway inhibitors. a colocalization assay of the major capsid protein vp39 with the early endosome marker eea1 showed that at low ph, acmnpv entered sf9 cells via an endosome-independent pathway. using a fluorescent probe (r18), we showed that at low ph, the viral nucleocapsid entered sf9 cells via direct fusion at the cell surface. by using the myosin-specific inhibitor 2,3-butanedione monoxime (bdm) and the microtubule inhibitor nocodazole, the low ph-triggered direct fusion was demonstrated to be dependent on myosin-like proteins and independent of microtubules. the reverse transcription-pcr of the ie1 gene as a marker for viral entry showed that the kinetics of acmnpv in cells triggered by low ph was similar to that of the normal entry via endocytosis. the low ph-mediated infection assay and vp39 and eea1 colocalization assay also demonstrated that acmnpv could efficiently transduce mammalian cells via direct membrane fusion at the cell surface. more importantly, we found that a low-ph trigger could significantly improve the transduction efficiency of acmnpv in mammalian cells, leading to the potential application of this method when using baculovirus as a vector for heterologous gene expression and for gene therapy.
WOS关键词	NUCLEAR POLYHEDROSIS-VIRUS ; CLATHRIN-MEDIATED ENDOCYTOSIS ; SEMLIKI-FOREST-VIRUS ; INSECT CELLS ; BACULOVIRUS VECTORS ; INFLUENZA-VIRUS ; GENE-DELIVERY ; RECOMBINANT BACULOVIRUS ; HUMAN HEPATOCYTES ; CATHEPSIN-L
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000277318600047
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375694
专题	武汉病毒研究所
通讯作者	Wang, Hualin
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Dong, Sicong,Wang, Manli,Qiu, Zhijuan,et al. Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph[J]. Journal of virology,2010,84(10):5351-5359.
APA	Dong, Sicong.,Wang, Manli.,Qiu, Zhijuan.,Deng, Fei.,Vlak, Just M..,...&Wang, Hualin.(2010).Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph.Journal of virology,84(10),5351-5359.
MLA	Dong, Sicong,et al."Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph".Journal of virology 84.10(2010):5351-5359.

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