Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph | |
Dong, Sicong1,2,4; Wang, Manli1,2; Qiu, Zhijuan1,2; Deng, Fei1,2; Vlak, Just M.3; Hu, Zhihong1,2; Wang, Hualin1,2 | |
刊名 | Journal of virology |
2010-05-01 | |
卷号 | 84期号:10页码:5351-5359 |
ISSN号 | 0022-538X |
DOI | 10.1128/jvi.02517-09 |
通讯作者 | Wang, hualin(h.wang@wh.iov.cn) |
英文摘要 | The budded virus (bv) of the autographa californica multicapsid nucleopolyhedrovirus (acmnpv) infects insect cells and transduces mammalian cells mainly through the endocytosis pathway. however, this study revealed that the treatment of the virus bound to sf9 cells at low ph could efficiently rescue the infectivity of acmnpv in the presence of endocytosis pathway inhibitors. a colocalization assay of the major capsid protein vp39 with the early endosome marker eea1 showed that at low ph, acmnpv entered sf9 cells via an endosome-independent pathway. using a fluorescent probe (r18), we showed that at low ph, the viral nucleocapsid entered sf9 cells via direct fusion at the cell surface. by using the myosin-specific inhibitor 2,3-butanedione monoxime (bdm) and the microtubule inhibitor nocodazole, the low ph-triggered direct fusion was demonstrated to be dependent on myosin-like proteins and independent of microtubules. the reverse transcription-pcr of the ie1 gene as a marker for viral entry showed that the kinetics of acmnpv in cells triggered by low ph was similar to that of the normal entry via endocytosis. the low ph-mediated infection assay and vp39 and eea1 colocalization assay also demonstrated that acmnpv could efficiently transduce mammalian cells via direct membrane fusion at the cell surface. more importantly, we found that a low-ph trigger could significantly improve the transduction efficiency of acmnpv in mammalian cells, leading to the potential application of this method when using baculovirus as a vector for heterologous gene expression and for gene therapy. |
WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; CLATHRIN-MEDIATED ENDOCYTOSIS ; SEMLIKI-FOREST-VIRUS ; INSECT CELLS ; BACULOVIRUS VECTORS ; INFLUENZA-VIRUS ; GENE-DELIVERY ; RECOMBINANT BACULOVIRUS ; HUMAN HEPATOCYTES ; CATHEPSIN-L |
WOS研究方向 | Virology |
WOS类目 | Virology |
语种 | 英语 |
出版者 | AMER SOC MICROBIOLOGY |
WOS记录号 | WOS:000277318600047 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2375694 |
专题 | 武汉病毒研究所 |
通讯作者 | Wang, Hualin |
作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 3.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Dong, Sicong,Wang, Manli,Qiu, Zhijuan,et al. Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph[J]. Journal of virology,2010,84(10):5351-5359. |
APA | Dong, Sicong.,Wang, Manli.,Qiu, Zhijuan.,Deng, Fei.,Vlak, Just M..,...&Wang, Hualin.(2010).Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph.Journal of virology,84(10),5351-5359. |
MLA | Dong, Sicong,et al."Autographa californica multicapsid nucleopolyhedrovirus efficiently infects sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low ph".Journal of virology 84.10(2010):5351-5359. |
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