ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2
	Chang, Guanxiao 1; Yang, Wenjuan 2; Zhang, Qili 2; Huang, Jinling3,4 ; Yang, Yongping1 ; Hu, Xiangyang 1,2
刊名	PLANT PHYSIOLOGY
	2019-02-01
卷号	179 期号:2 页码:477-490
ISSN号	0032-0889
英文摘要	ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown. Here, we found that flowering time was markedly delayed and CONSTANS (CO) expression was reduced in a transgenic Arabidopsis line overexpressing AFP2 under LD conditions. Conversely, the loss-of-function afp2 mutant showed slightly earlier flowering, with higher CO expression. These data suggest that AFP2 negatively regulates photoperiod-dependent flowering time by modulating the CO signal. We then found that AFP2 exhibited circadian expression rhythms that peaked during the night. Furthermore, the C-terminus of AFP2 interacted with CO, while its N-terminal ethylene response factor-associated amphiphilic repression motif interacted with the transcriptional corepressor TOPLESS-related protein2 (TPR2). Thus, AFP2 bridges CO and TPR2 to form the CO-AFP2-TPR2 complex. Biochemical and genetic analyses showed that AFP2 mediated CO degradation during the night. AFP2 also recruited histone deacetylase activity at Flowering Locus T chromatin through its interaction with TPR2. Taken together, our results reveal an elaborate mechanism by which AFP2 modulates flowering time through coordinating the activity and stability of CO.
资助项目	Shanghai University ; National Natural Science Foundation of China[31470348] ; National Natural Science Foundation of China[31570279] ; Applied Basic Research Project of Yunnan Province[2016FA015] ; 13th Five-Year Informatization Plan of the Chinese Academy of Sciences[XXH13506]
语种	英语
资助机构	Shanghai University ; National Natural Science Foundation of China ; Applied Basic Research Project of Yunnan Province ; 13th Five-Year Informatization Plan of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/64195]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Hu, Xiangyang
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 2.Shanghai Univ, Res Ctr Nat Prod, Shanghai Key Lab Bioenergy Crops, Plant Sci Ctr,Sch Life Sci, Shanghai 200444, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475001, Peoples R China 4.East Carolina Univ, Dept Biol, Greenville, NC 27858 USA
推荐引用方式 GB/T 7714	Chang, Guanxiao,Yang, Wenjuan,Zhang, Qili,et al. ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2[J]. PLANT PHYSIOLOGY,2019,179(2):477-490.
APA	Chang, Guanxiao,Yang, Wenjuan,Zhang, Qili,Huang, Jinling,Yang, Yongping,&Hu, Xiangyang.(2019).ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2.PLANT PHYSIOLOGY,179(2),477-490.
MLA	Chang, Guanxiao,et al."ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2".PLANT PHYSIOLOGY 179.2(2019):477-490.