Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize

doi:10.1021/pr200333f

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize
	Bai, Xuegui 1; Yang, Liming 1,2; Yang, Yunqiang1 ; Ahmad, Parvaiz 3; Yang, Yongping1 ; Hu, Xiangyang 1
刊名	JOURNAL OF PROTEOME RESEARCH
	2011-10-01
卷号	10 期号:10 页码:4349-4364
关键词	Maize Nitric Oxide Ros Saline Stress
ISSN号	1535-3893
DOI	10.1021/pr200333f
通讯作者	yangyp@mail.kib.ac.cn ; huxiangyang@mail.kib.ac.cn
文献子类	Article
英文摘要	Saline stress is a major factor that limits crop yield. Nitric oxide (NO) is functional during plant growth, development, and defense responses. In the present study, the protective role of NO in alleviating saline stress in maize at the physiological and proteomic levels was examined. Our results showed that salt treatment quickly induced NO accumulation and addition of the NO donor S-nitroso-N-acetylpenicillamine (SNAP) efficiently eliminated the inhibitory effect of salt on shoot growth and photosynthesis and inhibited salt-inducible H(2)O(2) accumulation. These effects could be reversed by NO metabolic scavengers and inhibitors. Further proteomic and Western blotting analysis revealed that NO induced G-protein-associated protein accumulation and antioxidant enzymes activities, in addition to activation of defense proteins, energy metabolism, and cell structure/division in salt-treated maize seedlings. Controlling the G-protein status with G-protein activators or inhibitors also affected NO generation and root and stem growth in maize seedlings after saline stress. On the basis of these results, we propose that NO enhances salt tolerance in maize seedlings by enhancing antioxidant enzyme activities and controlling H(2)O(2) levels, and these effects are accompanied by diverse downstream defense responses. During this process, G-protein signaling is an early event that works upstream of NO biogenesis.
学科主题	Biochemical Research Methods
WOS关键词	PROTEIN-KINASE CASCADE ; HYDROGEN-PEROXIDE ; ABSCISIC-ACID ; GLUTATHIONE-REDUCTASE ; PHOSPHOLIPASE-D ; NADPH OXIDASE ; PLANTS ; DEFENSE ; LEAVES ; SIGNAL
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000295602700001
资助机构	CAS; Major State Basic Research Development Program[2010CB951700]; National Science Foundation of China[30871704, 30971452, 31170256, 30900871]
公开日期	2012-03-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/3830]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China 3.Univ Kashmir, Dept Bot, AS Coll, Srinagar 190008, Jammu & Kashmir, India
推荐引用方式 GB/T 7714	Bai, Xuegui,Yang, Liming,Yang, Yunqiang,et al. Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(10):4349-4364.
APA	Bai, Xuegui,Yang, Liming,Yang, Yunqiang,Ahmad, Parvaiz,Yang, Yongping,&Hu, Xiangyang.(2011).Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize.JOURNAL OF PROTEOME RESEARCH,10(10),4349-4364.
MLA	Bai, Xuegui,et al."Deciphering the Protective Role of Nitric Oxide against Salt Stress at the Physiological and Proteomic Levels in Maize".JOURNAL OF PROTEOME RESEARCH 10.10(2011):4349-4364.