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科研机构
湖南农业大学 [10]
内容类型
期刊论文 [10]
发表日期
2017 [1]
2014 [2]
2013 [1]
2011 [2]
2010 [4]
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内容类型:期刊论文
专题:湖南农业大学
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Lead induces apoptosis in mouse TM3 Leydig cells through the Fas/FasL death receptor pathway
期刊论文
Environmental Toxicology and Pharmacology, 2017, 卷号: 56, 页码: 99-105
作者:
He, Xiuyuan
;
Wu, Jing*
;
Yuan, Liyun
;
Lin, Feng*
;
Yi, Jine
收藏
  |  
浏览/下载:10/0
  |  
提交时间:2019/12/23
Lead
TM3 leydig cells
Cytotoxicity
Apoptosis
Fas/FasL
Caspase-8
Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice
期刊论文
Journal of veterinary science, 2014, 卷号: 15, 期号: 1, 页码: 141-148
作者:
Yi, Jine*
;
Xia, Wei
;
Wu, Jianping
;
Yuan, Liyun
;
Wu, Jing
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/23
alcohol
antioxidant capacity
betulinic acid
lipid peroxidation
liver damage
Arsanilic acid causes apoptosis and oxidative stress in rat kidney epithelial cells (NRK-52e cells) by the activation of the caspase-9 and -3 signaling pathway
期刊论文
Drug and Chemical Toxicology, 2014, 卷号: 37, 期号: 1, 页码: 55-62
作者:
Lu, Yin
;
Yuan, Hui
;
Deng, Sijun
;
Wei, Qiang
;
Guo, Chengzhi
收藏
  |  
浏览/下载:8/0
  |  
提交时间:2019/12/23
Apoptosis
Arsanilic acid
Caspase-3 and -9
Mitochondria
NRK-52e cells
Reactive oxygen species
Gossypol acetic acid induces apoptosis in RAW264.7 cells via a caspase-dependent mitochondrial signaling pathway
期刊论文
Journal of veterinary science (Suwŏn-si, Korea), 2013, 卷号: 14, 期号: 3, 页码: 281-289
作者:
Deng, Sijun
;
Yuan, Hui*
;
Yi, Jine
;
Lu, Yin
;
Wei, Qiang
收藏
  |  
浏览/下载:4/0
  |  
提交时间:2019/12/23
apoptosis
gossypol acetic acid
mitochondrial signaling pathway
DNA damage and decrease of cellular oxidase activity in piglet Sertoli cells exposed to arsanilic acid.
期刊论文
The Journal of veterinary medical science, 2011, 卷号: 73, 期号: 2, 页码: 199-203
作者:
Zhang, Ming
;
He, Zuping
;
Yuan, Hui*
;
Zhu, Li
;
Guo, Chengzhi
收藏
  |  
浏览/下载:3/0
  |  
提交时间:2019/12/23
arsanilic acid
DNA damage
oxidase activity
piglet Sertoli cells
toxicity
DNA damage and decrease of cellular oxidase activity in piglet sertoli cells exposed to gossypol
期刊论文
African Journal of Biotechnology, 2011, 卷号: 10, 期号: 14, 页码: 2797-2802
作者:
Zhang, Ming
;
Yuan, Hui*
;
He, Zuping
;
Yuan, Liyun
;
Yi, Jine
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/23
Gossypol
sertoli cells
oxidase
DNA damage
Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure
期刊论文
Reproductive Biology and Endocrinology, 2010, 卷号: 8, 期号: 1, 页码: 97
作者:
Zhang, Ming
;
He, Zuping*
;
Wen, Lixin
;
Wu, Jing
;
Yuan, Liyun
收藏
  |  
浏览/下载:1/0
  |  
提交时间:2019/12/23
Cadmium
Male Germ Cell
Seminiferous Tubule
Sertoli Cell
Spermatogonial Stem Cell
Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice
期刊论文
Transgenic Research, 2010, 卷号: 19, 期号: 5, 页码: 809-818
作者:
Haiwen Zhang
;
Wu Liu
;
Liyun Wan
;
Fang Li
;
Liangying Dai
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/23
Drought
ERF protein JERF3
Osmotic stress
Rice
Transcriptional regulation
Gynostemma pentaphyllum protects mouse male germ cells against apoptosis caused by zearalenone via Bax and Bcl-2 regulation
期刊论文
Toxicology Mechanisms and Methods, 2010, 卷号: 20, 期号: 3, 页码: 153-158
作者:
Yuan, Hui
;
Deng, Youtian
;
Yuan, Liyun
;
Wu, Jing
;
Yuan, Zhihang
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/23
Gynostemma pentaphyllum
anti-toxicity
Zearalenone
male germ cells
Bax and Bcl-2
Generation and characterization of a monoclonal antibody to penicillic acid from Penicillium cyclopium
期刊论文
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 卷号: 9, 期号: 21, 页码: 3026-3031
作者:
Lei, Hongyu
;
He, Zuping
;
Yuan, Hui*
;
Wu, Jing
;
Wen, Lixin
收藏
  |  
浏览/下载:6/0
  |  
提交时间:2019/12/23
Penicillic acid is one of the main mycotoxins in moldy feedstuff and has toxic effect on livestock and poultry and probably humans due to food chain transmission. The objective of this study was to generate and characterize a monoclonal antibody to penicillic acid for the efficient detection of penicillic acid from Penicillium cyclopiumby immunological methods. To this end
penicillic acid was conjugated to bovine serum album (BSA) using the Mannich reaction and coupled with ovalbumin (OVA) by the method of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC) to generate artificial antigens penicillic acid-BSA and penicillic acid-OVA. A hybridoma cell line was obtained after fusion of mouse SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with artificial antigen penicillic acid-BSA. A monoclonal antibody specific against penicillic acid was produced in vivo by this hybridoma cell line. Further analysis revealed that the monoclonal antibody to penicillic acid was of the IgG1 subtype
with a titer of 1: 2.05 × 105. The antibody to penicillic acid had no or less cross-reaction with mycotoxins
including aflatoxin B1
zearalenone
T-2 toxin and fumonisins and more importantly
it assumed an affinity of about 1.54 × 108 liters per mol. Our ability to produce a monoclonal antibody to penicillic acid provides necessary groundwork for the effective detection of penicillic acid in various tissues of animals and human
using the immunocytochemistry
Western blots and enzyme-linked immunosorbent assay (ELISA).
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