Clinical application of a novel sliver nanoparticles biosensor based on localized surface plasmon resonance for detecting the microalbuminuria

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	Clinical application of a novel sliver nanoparticles biosensor based on localized surface plasmon resonance for detecting the microalbuminuria
	Lai, Ting 1; Hou, Qiannan 1; Yang, Huan 2; Luo, Xiangang 2; Xi, Mingrong 1
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2010-11-01
卷号	42 期号:11 页码:787-792
关键词	localized surface plasmon resonance (LSPR) biosensor microalbuminuria
英文摘要	In order to explore the clinical application of the nanobiosensor based on localized surface plasmon resonance (LSPR), we used our LSPR biosensor to detect the microalbuminuria in this work. The sliver nanoparticles were fabricated by using nanosphere lithography. The anti-human albumin antibody was immobilized on the sensor surface by amine coupling method. The different concentrations of commercial albumin and albumin in urine samples from three mild preeclampsia patients were determined according to the peak of LSPR extinction spectra. Under optimum conditions, our results showed that the biosensor displayed a detection limit of 1 ng/ml and wide dynamic range of 1 ng/ml to 1 mu g/ml. Furthermore, the microalbuminuria of three patients was determined by our biosensor within a short assay time, without sample purification. This biosensor proposed herein is easy to prepare and could be used for low-cost, rapid, label-free, and sensitive screening of the microalbuminuria. This approach provides a promising platform for developing clinical diagnostic applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NANOSCALE OPTICAL BIOSENSOR ; CARDIOVASCULAR EVENTS ; ALZHEIMERS-DISEASE ; REAL-TIME ; ALBUMINURIA ; BINDING ; RISK ; DEPENDENCE ; MORTALITY ; ASSAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000283657600004
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.ioe.ac.cn/handle/181551/3358]
专题	光电技术研究所_光电技术研究所被WoS收录文章
作者单位	1.Sichuan Univ, W China Univ Hosp 2, Dept Gynecol & Obstet, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Inst Opt & Elect, State Key Lab Opt Technol Microfabricat, Chengdu 610209, Peoples R China
推荐引用方式 GB/T 7714	Lai, Ting,Hou, Qiannan,Yang, Huan,et al. Clinical application of a novel sliver nanoparticles biosensor based on localized surface plasmon resonance for detecting the microalbuminuria[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2010,42(11):787-792.
APA	Lai, Ting,Hou, Qiannan,Yang, Huan,Luo, Xiangang,&Xi, Mingrong.(2010).Clinical application of a novel sliver nanoparticles biosensor based on localized surface plasmon resonance for detecting the microalbuminuria.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,42(11),787-792.
MLA	Lai, Ting,et al."Clinical application of a novel sliver nanoparticles biosensor based on localized surface plasmon resonance for detecting the microalbuminuria".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 42.11(2010):787-792.