Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)

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	Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)
	Sun, Bo-guang; Hu, Yong-hua
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2015-12-01
卷号	41 期号:6 页码:1463-1473
关键词	Cathepsin L Sciaenops ocellatus Teleost fish Red drum
英文摘要	Cathepsin L is a cysteine protease with a papain-like structure. It is known to be implicated in multiple processes of mammalian immune response to pathogen infection. In teleost fish, the functionality of cathepsin L is less understood. In this work, we characterized a cathepsin L homologue (designated as SoCatL) from red drum Sciaenops ocellatus, an important farmed fish species in China. SoCatL possesses a typical domain arrangement characteristic of cathepsin L, which comprises a proregion and a protease domain with four catalytically essential residues (Gln137, Cys143, His282 and Asn302) conserved in various organisms. SoCatL shares moderate sequence identities with mammalian cathepsin L and relatively high sequence identities with teleost cathepsin L. Phylogenetic analysis revealed that SoCatL is evolutionally close to fish cathepsin L, especially those belonging to the Perciformes order. The homology model of SoCatL was discovered to exhibit a structure resembling human cathepsin L. Transcriptional expression of SoCatL was found ubiquitous in tissues and enhanced after experimental infection with a bacterial pathogen. Recombinant SoCatL purified from Escherichia coli (designated as rSoCatL) displayed apparent proteolytic activity, which was optimal at 50 A degrees C and pH 7.0. The activity of rSoCatL required the catalytic residue Cys143 and was severely reduced by cathepsin inhibitor. These results suggest that SoCatL is a teleost cathepsin L homologue which functions as a cysteine protease and is likely to participate in the host immune response against bacterial infection.
收录类别	SCI
语种	英语
WOS记录号	WOS:000364517600009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/82293]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Bo-guang,Hu, Yong-hua. Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(6):1463-1473.
APA	Sun, Bo-guang,&Hu, Yong-hua.(2015).Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(6),1463-1473.
MLA	Sun, Bo-guang,et al."Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.6(2015):1463-1473.