Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)

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	Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)
	Jiang, Wei-Dan 1,2,3; Tang, Ren-Jun 1; Liu, Yang 1,2,3; Kuang, Sheng-Yao 4; Jiang, Jun 1,2,3; Wu, Pei 1,2,3; Zhao, Juan 1; Zhang, Yong-An 5; Tang, Ling 4; Tang, Wu-Neng 4
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-10-01
卷号	46 期号:2 页码:406-416
关键词	Manganese Cytokine Tight junction Antioxidant status TOR Nrf2
ISSN号	1050-4648
英文摘要	Intestinal mucosal immune components and mRNA levels of inflammatory cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules in young grass carp (Ctenopharyngodon idellus) under dietary manganese (Mn) deficiency or excess were investigated. Fish were fed the diets containing graded levels of Mn [3.65-27.86 mg Mn kg(-1) diet] for 8 weeks. The results demonstrated that Mn deficiency significantly decreased the lysozyme and acid phosphatase (ACP) activities, up-regulated tumour necrosis factor alpha (TNF-alpha), interleukin 8 and the signalling factor nuclear factor-kappa B p65, and down-regulated interleukin 10 (IL-10), transforming growth factor beta 1, inhibitor of signalling factors kappa B-alpha and target of rapamycin mRNA levels in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI). However, Mn deficiency did not change the C3 content in the PI, whereas it decreased the C3 contents in the MI and DI. Additionally, Mn depletion also resulted in significantly low mRNA levels for tight junction proteins (claudin-b, claudin-c, claudin-15, occludin and zonula occludens-1), antioxidant enzymes (MnSOD, GPx and CAT) and NF-E2-related factor-2 in the intestines of fish. Excessive Mn exhibited toxic effects similar to Mn deficiency, where optimal Mn contents reversed those indicators. In conclusion, Mn deficiency or excess causes the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier relating to NF-kappa B, TOR and Nrf2 signalling in grass carp. Furthermore, quadratic regression analysis at 95% maximum response of lysozyme and acid phosphatase activities in the distal intestine of young grass carp revealed the optimum dietary Mn levels to be 8.90 and 8.99 mg kg(-1) diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	SALMON SALMO-SALAR ; TIGHT JUNCTION PERMEABILITY ; TROUT ONCORHYNCHUS-MYKISS ; ATLANTIC SALMON ; DIETARY MANGANESE ; GENE-EXPRESSION ; MITOCHONDRIAL DYSFUNCTION ; AEROMONAS-SALMONICIDA ; OREOCHROMIS-NILOTICUS ; TRANSCRIPT ABUNDANCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000363071200028
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27329]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Sichuan Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Wei-Dan,Tang, Ren-Jun,Liu, Yang,et al. Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):406-416.
APA	Jiang, Wei-Dan.,Tang, Ren-Jun.,Liu, Yang.,Kuang, Sheng-Yao.,Jiang, Jun.,...&Feng, Lin.(2015).Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,46(2),406-416.
MLA	Jiang, Wei-Dan,et al."Manganese deficiency or excess caused the depression of intestinal immunity, induction of inflammation and dysfunction of the intestinal physical barrier, as regulated by NF-kappa B, TOR and Nrf2 signalling, in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):406-416.