SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes

	SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes
	Sun, Liangliang 1; Zhang, Yanyan 2; Tao, Dingyin 1; Zhu, Guijie 1; Zhao, Qun 1,3; Wu, Qi 1,3; Liang, Zhen 1; Yang, Ling 2; Zhang, Lihua 1; Zhang, Yukui 1
刊名	proteomics
	2012-12-01
卷号	12 期号:23-24 页码:3464-3469
关键词	1-Butyl-3-methyl imidazolium tetrafluoroborate 2D-LC-ESI-MS/MS Cytochrome P450 enzyme Protein spectral count Uridine diphosphoglucuronosyl transferase
英文摘要	comprehensive identification of cytochrome p450 enzymes (cyps) and uridine diphosphoglucuronosyl transferases (ugts) in human liver microsomes (hlms) was performed with an sds-page-free protocol. hlms were solubilized with 5% v/v ionic liquid, 1-butyl-3-methyl imidazolium tetrafluoroborate, followed by tryptic digestion, and 2d-scx-rplc-esi-ms/ms (ltq xl) analysis in triplicate. in total, 27 cyps and 12 ugts were confidently identified with average sequence coverage as 30.99 and 25.07%, average peptide number as 14 and 13, and average unique peptide number as 7 and 4, respectively. the highly similar isoforms of cyp3a, cyp2c, and cyp4f subfamilies could be unambiguously differentiated from each other, despite the fact that the sequence similarity of cyp2c9 and cyp2c19 is 91%. in addition, protein spectral count was used to approximately evaluate the relative abundance of identified cyps and ugts, and the results agreed with previous immunochemistry reports.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	tandem mass-spectrometry ; proteomic approach ; isoforms ; p450 ; quantification ; therapeutics ; expression ; tissue
收录类别	SCI
语种	英语
WOS记录号	WOS:000312650100003
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/143063]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab High Efficient Separat & High Sensit Characte, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Liangliang,Zhang, Yanyan,Tao, Dingyin,et al. SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes[J]. proteomics,2012,12(23-24):3464-3469.
APA	Sun, Liangliang.,Zhang, Yanyan.,Tao, Dingyin.,Zhu, Guijie.,Zhao, Qun.,...&Zhang, Yukui.(2012).SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes.proteomics,12(23-24),3464-3469.
MLA	Sun, Liangliang,et al."SDS-PAGE-free protocol for comprehensive identification of cytochrome P450 enzymes and uridine diphosphoglucuronosyl transferases in human liver microsomes".proteomics 12.23-24(2012):3464-3469.