Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry

	Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry
	Qiao, Xiaoqiang 1,2; Sun, Liangliang 1,2; Chen, Lingfan 1,2; Zhou, Yuan 1,2; Yang, Kaiguang 1; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	rapid communications in mass spectrometry
	2011-03-15
卷号	25 期号:5 页码:639-646
英文摘要	piperazine-based derivatives, including 1-(2-pyridyl) piperazine (2-pp), 1-(2-pyrimidyl) piperazine (2-pmp), 1-(4-pyridyl) piperazine (4-pp), and 1-(1-methyl-4-piperidinyl) piperazine (m-pp), were used for the derivatization of carboxyl groups on peptides with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (edc) and 1-hydroxy-7-azabenzotriazole (hoat) as coupling reagents, and trifluoroacetic acid (tfa) as activator. taking synthetic peptides rvyvhpi (ri-7) and apgdriyvhpf (af-11) as samples, the yields of derivatized peptides by 2-pp, 2-pmp and 4-pp were higher than 94%. the effect of piperazine derivatives on the signals of tryptic digests of a-transferrin and bovine serum albumin (bsa) was investigated, and it was found that peptides derivatized by 2-pp and 2-pmp exhibited obviously improved ionization efficiency. furthermore, comparison of identified peptides before and after derivatization showed that peptides with low molecular weight (mw) and high pi value were preferably detected after derivatization. in addition, after derivatization with 2-pp and 2-pmp, protein myelin basic protein s, 20 kda protein, and histone h were confidently identified from the tryptic digests of two fractions of rat brain protein separated by reversed-phase high-performance liquid chromatography (hplc), indicating the potential application of 2-pp and 2-pmp for the highly sensitive determination of peptides in comprehensive proteome analysis. copyright (c) 2011 john wiley & sons, ltd.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences ; technology
类目[WOS]	biochemical research methods ; chemistry, analytical ; spectroscopy
研究领域[WOS]	biochemistry & molecular biology ; chemistry ; spectroscopy
关键词[WOS]	electrospray-ionization efficiency ; amino-groups ; maldi ; phosphopeptides ; sensitivity ; proteomics ; protein ; ms
收录类别	SCI
语种	英语
WOS记录号	WOS:000287594900010
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142655]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Xiaoqiang,Sun, Liangliang,Chen, Lingfan,et al. Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry[J]. rapid communications in mass spectrometry,2011,25(5):639-646.
APA	Qiao, Xiaoqiang.,Sun, Liangliang.,Chen, Lingfan.,Zhou, Yuan.,Yang, Kaiguang.,...&Zhang, Yukui.(2011).Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry.rapid communications in mass spectrometry,25(5),639-646.
MLA	Qiao, Xiaoqiang,et al."Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry".rapid communications in mass spectrometry 25.5(2011):639-646.