Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde

	Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde
	Liu, Xing-yu 1,2; Yang, Zhi-hua 3; Pan, Xiu-jie 3; Zhu, Mao-xiang 3; Xie, Jian-ping 1
刊名	toxicology letters
	2010-08-16
卷号	197 期号:2 页码:113-122
关键词	Crotonaldehyde Gene expression profile Microarray
英文摘要	crotonaldehyde is an environment pollutant and lipid peroxidation product crotonaldehyde produces adverse effects to humans and serves as a risk factor for human pulmonary diseases like acrolein and 4-hydroxynonenal, crotonaldehyde seems likely to alter many cell signaling cascades, including inflammatory responses the purpose of this study was to investigate the genome-wide transcriptional responses of normal human bronchial epithelial cells exposed to crotonaldehyde using microarrays technology, the global changes in transcriptional level were analyzed. prior to rna extraction, cells were exposed to crotonaldehyde at 40 or 80 mu m for 3 or 6 h real-time quantitative polymerase chain reaction (qpcr) was performed to validate microarray data and cell cycle arrest was determined the commonly differentially regulated genes in many biological processes were dysregulated including inflammatory responses, exogenous metabolism, cell cycle, heat shock responses, and antioxidant responses results in the present study screen out the important roles of hmox1 in regulating other signaling cascades and aldh1a3 in detoxifying exogenous toxicants collectively, our study demonstrated that crotonaldehyde altered gene expression profile in the genome-wide transcriptional level in normal human bronchial epithelial cells. and many of them represented potential mechanisms of crotonaldehyde causing cytotoxicity and tissue injury in the human lung (c) 2010 elsevier ireland ltd all rights reserved
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	toxicology
研究领域[WOS]	toxicology
关键词[WOS]	liver aldehyde dehydrogenases ; kappa-b activation ; cigarette-smoke ; alpha,beta-unsaturated aldehydes ; lipid-peroxidation ; oxidative stress ; a549 cells ; transcriptional responses ; acrolein toxicity ; heme oxygenase-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000280052200008
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141864]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou 450001, Henan, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China 3.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xing-yu,Yang, Zhi-hua,Pan, Xiu-jie,et al. Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde[J]. toxicology letters,2010,197(2):113-122.
APA	Liu, Xing-yu,Yang, Zhi-hua,Pan, Xiu-jie,Zhu, Mao-xiang,&Xie, Jian-ping.(2010).Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde.toxicology letters,197(2),113-122.
MLA	Liu, Xing-yu,et al."Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde".toxicology letters 197.2(2010):113-122.