Lysophosphatidylcholine profiling of plasma: discrimination of isomers and discovery of lung cancer biomarkers

	Lysophosphatidylcholine profiling of plasma: discrimination of isomers and discovery of lung cancer biomarkers
	Dong, Jun 1,2; Cai, Xiaoming 2; Zhao, Lili 1; Xue, Xingya 2; Zou, Lijuan 1; Zhang, Xiuli 2; Liang, Xinmiao 2
刊名	metabolomics
	2010-12-01
卷号	6 期号:4 页码:478-488
关键词	Lysophosphatidylcholine Isomers Discrimination Metabonomics Lung cancer
英文摘要	lysophosphatidylcholines (lysopcs) are a class of compounds that have a constant polar head, and fatty acyls of different chain lengths, position, degrees of saturation, and double bond location in human plasma. lysopcs levels can be a clinical diagnostic indicator that reveals pathophysiological changes. in this work, a method was developed to discriminate between different types of lysopcs using reversed phase ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry, using mass spectrometry ms(e). isomeric lysopcs were distinguished based on retention time and the peak intensity ratio of product ions, and 14 pairs of lysopcs regioisomers were identified in human plasma. the plasma samples of 12 lung cancer patients and 12 healthy persons were collected and analyzed by principal component analysis to generate metabolic profiles of the identified lysopcs. both electrospray ionization esi+ and esi- results showed that all lung cancer patients had the same five lysopc metabolic abnormalities, specifically in sn-1 lyso16:0, sn-2 lysopc 16:0, sn-1 lysopc 18:0, sn-1 lysopc 18:1 and sn-1 lysopc 18:2. thus, the function of isomers with different fatty acyl positions may be related to lung cancer, and this may help elucidate the mechanism of the disease.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	endocrinology & metabolism
研究领域[WOS]	endocrinology & metabolism
关键词[WOS]	uplc-oatof-ms ; chromatography-mass spectrometry ; diabetes-mellitus ; metabonomics research ; potential biomarkers ; metabolism ; rat ; lysophospholipids ; phosphatidylcholine ; cells
收录类别	SCI
语种	英语
WOS记录号	WOS:000283944300002
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141708]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Dong, Jun,Cai, Xiaoming,Zhao, Lili,et al. Lysophosphatidylcholine profiling of plasma: discrimination of isomers and discovery of lung cancer biomarkers[J]. metabolomics,2010,6(4):478-488.
APA	Dong, Jun.,Cai, Xiaoming.,Zhao, Lili.,Xue, Xingya.,Zou, Lijuan.,...&Liang, Xinmiao.(2010).Lysophosphatidylcholine profiling of plasma: discrimination of isomers and discovery of lung cancer biomarkers.metabolomics,6(4),478-488.
MLA	Dong, Jun,et al."Lysophosphatidylcholine profiling of plasma: discrimination of isomers and discovery of lung cancer biomarkers".metabolomics 6.4(2010):478-488.