Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid

	Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid
	Cao, Qing 1; Quan, Li 1; He, Chiyang 2; Li, Na 1; Li, Kean 1; Liu, Feng 1
刊名	talanta
	2008-10-15
卷号	77 期号:1 页码:160-165
关键词	Horseradish peroxidase Enzyme activity Partition Ionic liquid Aqueous biphasic system
英文摘要	enzyme activity and partition behavior in aqueous biphasic systems (abss) consisting of ionic liquid (il) and salt (il-abss) were investigated to increase our understanding of il-abss and shed light on their application potential as enzyme extraction system. with horseradish peroxidase (hrp) as the model enzyme, several effects of alkylimidazolium chloride-k2hpo4 abss on activity and partition behavior of enzyme were studied including alkyl chain length of ils and concentrations of each component. high lyotropic ils (1-butyl-3-methylimidazolium and 1-ethyl-3-methylimidazolium chloride) and adequate water content (>40%) were both essential for the activity maintenance of hrp in il-abs. 1-butyl-3-methylimidazolium chloride ([c(4)mim]cl) was found to be an appropriate il for phase forming and hrp activity retaining. after optimization of phase condition, about 80% hrp amount was distributed in the il-rich upper phase, and greater than 90% enzyme activity was obtained. moreover, compared with the commonly used polymer-based abss, this [c(4)mim]cl-abs has a much lower viscosity, which is very beneficial to the experimental operation. therefore, the tested il-abs could be considered as a potential enzyme extraction system. (c) 2008 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	antarctica lipase-b ; 2-phase system ; solvent-extraction ; crown-ether ; separation ; water ; salt ; selectivity ; stability ; media
收录类别	SCI
语种	英语
WOS记录号	WOS:000260290200024
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140956]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 2.Anqing Normal Coll, Dept Chem, Anqing 246011, Peoples R China
推荐引用方式 GB/T 7714	Cao, Qing,Quan, Li,He, Chiyang,et al. Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid[J]. talanta,2008,77(1):160-165.
APA	Cao, Qing,Quan, Li,He, Chiyang,Li, Na,Li, Kean,&Liu, Feng.(2008).Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid.talanta,77(1),160-165.
MLA	Cao, Qing,et al."Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid".talanta 77.1(2008):160-165.