Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells

	Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells
	Yu, WT ; Liu, XD ; Li, XX ; Qi, WT ; Ren, DW ; Ma, XJ
刊名	chemical journal of chinese universities-chinese
	2006-01-10
卷号	27 期号:1 页码:182-186
关键词	chitosan alginate pH microcapsule cell culture
英文摘要	sodium alginate-chitosan (ac) microcapsules are often used as the immobilization carriers for cell culture. they are composed of porous gel as the core facilitating cell adhesion, and coated with polyelectrolyte complex membrane protecting cells and allowing selective mass transfer. therefore, the regulation and control of membrane properties can directly affect the growth and metabolism of cells in microcapsules. among many parameters, ph of chitosan solution is the important one. in this paper, the formation of polyelectrolyte membrane, the degree of complex formation and protein diffusion at different ph values were directly observed in situ by using a confocal laser scanning microscope(clsm). the effect of ph on the properties of ac microcapsule membrane was discussed. the optimal ph was determined in the preparation of ac microcapsules with different cells(animal and microbial cells). it was demonstrated that the membrane thickness showed "high-low-high" shape, the swelling behavior of microcapsules "low-high-low" shape and the protein diffusion across the membrane "low-high-low "shape as a function of ph, the inflection points all appeared at ph = 4.00 and 5.50. combining with the sensitivity experiment of different kinds of cells to environment ph, it was suggested that the optimal ph of chitosan solution for ac microencapsulated animal cells was 5.50, ac microencapsulated e. coli cells 5.00, and ac microencapsulated yeast cells such as saccharomyces cerevisiae (s. cere.) 4.50, respectively.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	behavior ; beads
收录类别	SCI
语种	英语
WOS记录号	WOS:000234815500047
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140267]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Sichuan Univ, Coll Mat Sci & Engn, Chengdu 610064, Peoples R China
推荐引用方式 GB/T 7714	Yu, WT,Liu, XD,Li, XX,et al. Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells[J]. chemical journal of chinese universities-chinese,2006,27(1):182-186.
APA	Yu, WT,Liu, XD,Li, XX,Qi, WT,Ren, DW,&Ma, XJ.(2006).Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells.chemical journal of chinese universities-chinese,27(1),182-186.
MLA	Yu, WT,et al."Effect of pH of chitosan solution on properties of sodium alginate-chitosan microcapsules with cells".chemical journal of chinese universities-chinese 27.1(2006):182-186.