Peptide separation in hydrophilic interaction capillary electrochromatography

	Peptide separation in hydrophilic interaction capillary electrochromatography
	Fu, HJ; Jin, WH; Xiao, H; Huang, HW; Zou, HF
刊名	electrophoresis
	2003-06-01
卷号	24 期号:12-13 页码:2084-2091
关键词	capillary electrochromatography hydrophilic interaction peptides separation mechanism strong-cation exchange
英文摘要	separation of small peptides by hydrophilic interaction capillary electrochromatography (hi-cec) has been investigated. the negative surface charge of a hydrophilic, strong-cation-exchange stationary phase (polysulfoethyl a) provided a substantial cathodic electroosmotic flow (eof). the influence of acetonitrile content, ionic strength, mobile phase ph as well as applied voltage on the migration of the peptides was studied. possible retention mechanisms of the peptides in hi-cec were discussed. it was found that hydrophilic interaction between the solutes and the stationary phase played a major role in this system, especially when mobile phases with high acetonitrile content were used. however, an ion-exchange mechanism and electrophoretic mobility also affect the migration of the peptides in hi-cec. elution order and selectivity was proved to be different in hi-cec and capillary zone electrophoresis (cze), thus revealing the potential of hi-cec as a complementary technique to cze for the separation of peptides. efficiency and selectivity of hi-cec for the separation of peptides were demonstrated by baseline separating nine peptides in 6 min.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; reversed-phase ; polar compounds ; electroendoosmotic chromatography ; exchange chromatography ; nucleic-acids ; proteins ; columns ; behavior ; driven
收录类别	SCI
语种	英语
WOS记录号	WOS:000184233300017
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139274]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Fu, HJ,Jin, WH,Xiao, H,et al. Peptide separation in hydrophilic interaction capillary electrochromatography[J]. electrophoresis,2003,24(12-13):2084-2091.
APA	Fu, HJ,Jin, WH,Xiao, H,Huang, HW,&Zou, HF.(2003).Peptide separation in hydrophilic interaction capillary electrochromatography.electrophoresis,24(12-13),2084-2091.
MLA	Fu, HJ,et al."Peptide separation in hydrophilic interaction capillary electrochromatography".electrophoresis 24.12-13(2003):2084-2091.