Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis

	Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis
	Li, W ; Zhang, D ; Lin, B ; Su, Z
刊名	chromatographia
	2000-10-01
卷号	52 期号:7-8 页码:451-454
关键词	column liquid chromatography size-exclusion chromatography capillary electrophoresis PEGlated hemoglobin
英文摘要	bovine hemoglobin (hb) has been chemically modified, by reaction of its lysine residues with the active ester of poly(ethylene glycol) (peg, m-w = 5000), to produce a potential blood substitute for human therapy. covalent attachment of peg chains to the protein produced a heterogeneous mixture of peglated hb (peg-hb). it is difficult but necessary to remove unmodified and poorly modified hb from the mixture. this paper describes the use of cation-exchange chromatography (iec), in flow-through mode, and size-exclusion chromatography (sec) for purification of the peg-hb mixture. the highly modified hb flowed through the iec column in the loading buffer without adsorption by the chromatographic medium. sec was then used for further purification. these two steps were suitable for pilot-scale preparation or for analytical chromatography. the purified product was assessed by high-performance capillary electrophoresis (hpce), which was also used to optimize the chromatographic parameters.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	polyethylene-glycol ; superoxide-dismutase
收录类别	SCI
语种	英语
WOS记录号	WOS:000165295100012
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138718]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Li, W,Zhang, D,Lin, B,et al. Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis[J]. chromatographia,2000,52(7-8):451-454.
APA	Li, W,Zhang, D,Lin, B,&Su, Z.(2000).Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis.chromatographia,52(7-8),451-454.
MLA	Li, W,et al."Purification and identification of PEGlated hemoglobin, a potential blood substitute, by chromatography and capillary electrophoresis".chromatographia 52.7-8(2000):451-454.