On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue

	On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue
	Yang, Qin; Shi, Xianzhe; Gu, Qun; Zhao, Sumin; Shan, Yuanhong; Xu, Guowang
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2012-05-01
卷号	895 页码:48-55
关键词	Comprehensive two dimensional liquid chromatography Trigacylglyceride Mouse liver Silver-ion LC APCI MS
英文摘要	triacylglycerides (tags) are a large class of complex neutral lipids that naturally occur in both plants and animals. in the present work, an on-line comprehensive silver-ion liquid chromatography (silver-ion lc) x reversed-phase liquid chromatography (rplc) system was constructed to analyze these compounds. a micro bore silver-ion modified column was employed in the first dimension with the commonly used hexane-based mobile phase. after a series of c18 columns were assessed, a wide bore column packed with 1.5 mu m particles was selected as the second dimension column to reduce the negative effect caused by the large volume and strong solvent injection in the second dimension. the system coupled with mass spectrometry was applied to the analysis of an edible peanut oil and a mouse liver extract. twenty-eight tags from the peanut oil and forty-four from the mouse liver were identified based on the tags' retention behaviors on the comprehensive two-dimensional lc system and their apci ms fragments. (c) 2012 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	pressure chemical-ionization ; electrospray mass-spectrometry ; evaporative light-scattering ; silver ion chromatography ; nonaqueous reversed-phase ; fluid chromatography ; positional isomers ; vegetable-oils ; rapid method ; olive oil
收录类别	SCI
语种	英语
WOS记录号	WOS:000303628700007
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138043]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yang, Qin,Shi, Xianzhe,Gu, Qun,et al. On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2012,895:48-55.
APA	Yang, Qin,Shi, Xianzhe,Gu, Qun,Zhao, Sumin,Shan, Yuanhong,&Xu, Guowang.(2012).On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue.journal of chromatography b-analytical technologies in the biomedical and life sciences,895,48-55.
MLA	Yang, Qin,et al."On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue".journal of chromatography b-analytical technologies in the biomedical and life sciences 895(2012):48-55.