Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-kappa B signal pathway

	Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-kappa B signal pathway
	Wang, Cai-Ping 1; Li, Jian-Long 1; Zhang, Lu-Zhong 1; Zhang, Xiao-Chuan 1; Yu, Shu 1; Liang, Xin-Miao 1,2; Ding, Fei 1; Wang, Zhi-Wei 1,3
刊名	neurochemistry international
	2013-12-01
卷号	63 期号:8 页码:741-749
关键词	Isoquercetin Oxygen-glucose deprivation followed by reperfusion (OGD/R) Ischemia Apoptosis Inflammation
英文摘要	in the present study, oxygen-glucose deprivation followed by reperfusion (ogd/r), an in vitro model of ischemia, was used to evaluate the neuroprotective effect of isoquercetin in primary culture of rat cortical neuronal cells. it was found that isoquercetin administered prior to the insult could prevent ogd/r-induced intracellular calcium concentrations ([ca2+](i)) increase, lactate dehydrogenase (ldh) release and cell viability decrease. for the first time, isoquercetin is described as a neuroprotective agent that potentially explains the alleviation and prevention from ogd/r-induced injury in neurons. mechanistic studies showed that the neuroprotective effect of isoquercetin was carried out by anti-inflammatory signaling pathway of inhibiting protein expression of toll-like receptor 4 (tlr4) and nuclear factor-kappa b (nf-kappa b), and mrna expression of tnf-alpha and il-6, accompanied by the anti-apoptotic signaling pathway of deactivation of extracellular-regulated kinase (erk), jun kinase (jnk) and p38, and inhibition of activity of caspase-3. therefore, these studies highlighted the confirmation of isoquercetin, a flavonoid compound, as an anti-inflammation and anti-apoptosis factor which might be used as a therapeutic strategy for the ischemia/reperfusion (i/r) brain injury and related diseases. (c) 2013 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; neurosciences
研究领域[WOS]	biochemistry & molecular biology ; neurosciences & neurology
关键词[WOS]	focal cerebral-ischemia ; toll-like receptor-4 ; nf-kappa-b ; experimental stroke ; brain-injury ; in-vitro ; terminal kinase ; mice ; cells ; inflammation
收录类别	SCI
语种	英语
WOS记录号	WOS:000328872300004
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137910]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nantong Univ, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Univ Calif Irvine, Dept Pharmacol, Irvine, CA 92697 USA
推荐引用方式 GB/T 7714	Wang, Cai-Ping,Li, Jian-Long,Zhang, Lu-Zhong,et al. Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-kappa B signal pathway[J]. neurochemistry international,2013,63(8):741-749.
APA	Wang, Cai-Ping.,Li, Jian-Long.,Zhang, Lu-Zhong.,Zhang, Xiao-Chuan.,Yu, Shu.,...&Wang, Zhi-Wei.(2013).Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-kappa B signal pathway.neurochemistry international,63(8),741-749.
MLA	Wang, Cai-Ping,et al."Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-kappa B signal pathway".neurochemistry international 63.8(2013):741-749.