Characterization of protoberberine alkaloids in Coptidis Rhizoma (Huanglian) by HPLC with ESI-MS/MS

	Characterization of protoberberine alkaloids in Coptidis Rhizoma (Huanglian) by HPLC with ESI-MS/MS
	Ren, Lingling 1,2; Xue, Xingya 1; Liang, Xinmiao 1
刊名	journal of separation science
	2013-04-01
卷号	36 期号:8 页码:1389-1396
关键词	Coptidis Rhizoma Chromatography retention parameters ESI-MS MS Protoberberine alkaloid
英文摘要	this study aims to qualitatively analyze protoberberine alkaloids in crude extract of coptidis rhizoma using hplc with esi-ms/ms. possible specific molecular weights of protoberberine alkaloids were firstly deduced according to literatures and were adopted to screen the alkaloids in the hplc with esi-ms of crude extract of coptidis rhizoma. as a result, 21 protoberberine alkaloids were found, including compounds of very low concentration and compounds coeluted in one peak. among these, two compounds were positively identified and verified by comparison with standards. ten of these compounds were first reported in this study for coptidis rhizoma. in addition, chromatographic retention parameters a and c of all compounds were obtained using their retention times under five gradient conditions and were applied to confirm the deduction about the structures of protoberberine alkaloids by tandem mass data.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; ionization-mass-spectrometry ; evodia herb couple ; chinese medicine ; capillary electrophoresis ; berberine ; palmatine ; plasma ; ms
收录类别	SCI
语种	英语
WOS记录号	WOS:000319866000009
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137863]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Anhui Med Univ, Dept Publ Hlth, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Ren, Lingling,Xue, Xingya,Liang, Xinmiao. Characterization of protoberberine alkaloids in Coptidis Rhizoma (Huanglian) by HPLC with ESI-MS/MS[J]. journal of separation science,2013,36(8):1389-1396.
APA	Ren, Lingling,Xue, Xingya,&Liang, Xinmiao.(2013).Characterization of protoberberine alkaloids in Coptidis Rhizoma (Huanglian) by HPLC with ESI-MS/MS.journal of separation science,36(8),1389-1396.
MLA	Ren, Lingling,et al."Characterization of protoberberine alkaloids in Coptidis Rhizoma (Huanglian) by HPLC with ESI-MS/MS".journal of separation science 36.8(2013):1389-1396.