Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation

	Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation
	Ma, Ying 1,2; Zhang, Ying 1; Liu, Yang 1; Chen, Li 1,2; Li, Shen 1,2; Zhao, Wei 1,2; Sun, Guangwei 1; Li, Na 1,2; Wang, Yu 1,2; Guo, Xin 1
刊名	journal of biomedical materials research part a
	2013-05-01
卷号	101 期号:5 页码:1265-1273
关键词	microencapsulation alginate poly-amino acid membrane E-poly-L-lysine cell culture
英文摘要	cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy, and large-scale cell culture. the well-studied -ap (alginate-poly-l-lysine) microcapsules have been restricted to large-scale cell-culture and clinical applications because of high costs and cytotoxic effects in some cases. this study used e-poly-l-lysine (e-pll), a high-biocompatible and low-cost food additives produced by fermentation, to prepare e-ap (alginatee-pll) microcapsules with various thickness membranes and swelling behaviors. e-ap microcapsules were permeable to bsa, a standard protein of culture medium. e-ap-microencapsulated chinese hamster ovary (cho) cells proliferated with culture time; no obvious difference with -ap-microencapsulated cho cells during the early 19 days. whereas e-ap-microencapsulated cho cells kept higher viability (od = 0.646 +/- 0.012) on the 22nd day and microcapsule strength (integrity rate of 88%) on the 24th day than that of -ap microcapsules (od = 0.558 +/- 0.025, integrity rate of 80%). e-ap (alginate-e-pll) microcapsules exhibited more superior properties and could lower the costs to broaden the applications of microencapsulation technology. (c) 2012 wiley periodicals, inc. j biomed mater res part a, 2013.
WOS标题词	science & technology ; technology
类目[WOS]	engineering, biomedical ; materials science, biomaterials
研究领域[WOS]	engineering ; materials science
关键词[WOS]	monoclonal-antibody production ; polycation microcapsules ; animal-cells ; stability ; growth ; permeability ; optimization ; technology ; delivery ; chitosan
收录类别	SCI
语种	英语
WOS记录号	WOS:000316815700005
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/137670]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ma, Ying,Zhang, Ying,Liu, Yang,et al. Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation[J]. journal of biomedical materials research part a,2013,101(5):1265-1273.
APA	Ma, Ying.,Zhang, Ying.,Liu, Yang.,Chen, Li.,Li, Shen.,...&Ma, Xiaojun.(2013).Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation.journal of biomedical materials research part a,101(5),1265-1273.
MLA	Ma, Ying,et al."Investigation of alginate-epsilon-poly-L-lysine microcapsules for cell microencapsulation".journal of biomedical materials research part a 101.5(2013):1265-1273.