Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes

CORC > 化学研究所 > 中国科学院化学研究所 > 分子纳米结构与纳米技术实验室

	Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes
	He, Kangmin 1,2,3,4; Yan, Xiaohua 5; Li, Nan 1; Dang, Song 1; Xu, Li 1; Zhao, Bing 5; Li, Zijian 2,3,4; Lv, Zhizhen 2,3,4; Fang, Xiaohong 1; Zhang, Youyi 2,3,4
刊名	CELL RESEARCH
	2015-06-01
卷号	25 期号:6 页码:738-752
关键词	clathrin caveolae TGF-beta receptor caveolin-1-positive early endosomes endocytic trafficking
英文摘要	Endocytosis and intracellular sorting of transforming growth factor-beta (TGF-beta) receptors play an important regulatory role in TGF-beta signaling. Two major endocytic pathways, clathrin-and caveolae-mediated endocytosis, have been reported to independently mediate the internalization of TGF-beta receptors. In this study, we demonstrate that the clathrin-and caveolae-mediated endocytic pathways can converge during TGF-beta receptor endocytic trafficking. By tracking the intracellular dynamics of fluorescently-labeled TGF-beta type I receptor (T beta RI), we found that after mediating T beta RI internalization, certain clathrin-coated vesicles and caveolar vesicles are fused underneath the plasma membrane, forming a novel type of caveolin-1 and clathrin double-positive vesicles. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized T beta RI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). We further showed that the caveolin-1-positive early endosomes are positive for Smad3/SARA, Rab11 and Smad7/Smurf2, and may act as a multifunctional device for TGF-beta signaling and TGF-beta receptor recycling and degradation. Therefore, these findings uncover a novel scenario of endocytosis, the direct fusion of clathrin-coated and caveolae vesicles during TGF-beta receptor endocytic trafficking, which leads to the formation of the multifunctional sorting device, caveolin-1-positive early endosomes, for TGF-beta receptors.
收录类别	SCI
语种	英语
公开日期	2015-11-02
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28175]
专题	化学研究所_分子纳米结构与纳米技术实验室
作者单位	1.Chinese Acad Sci, Nanotechnol Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China 3.Peking Univ, Acad Adv Interdisciplinary Studies, Key Lab Cardiovascular Mol Biol & Regulatory Pept, Minist Hlth,Key Lab Mol Cardiovasc Sci,Minist Edu, Beijing 100191, Peoples R China 4.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100191, Peoples R China 5.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	He, Kangmin,Yan, Xiaohua,Li, Nan,et al. Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes[J]. CELL RESEARCH,2015,25(6):738-752.
APA	He, Kangmin.,Yan, Xiaohua.,Li, Nan.,Dang, Song.,Xu, Li.,...&Chen, Ye-Guang.(2015).Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes.CELL RESEARCH,25(6),738-752.
MLA	He, Kangmin,et al."Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes".CELL RESEARCH 25.6(2015):738-752.