Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction

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	Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction
	He, Kangmin 1,2,3,4; Xing, Rui 1,2,3; Yan, Xiaohua 5; Tian, Aiju 1,2,3; Zhang, Mingliang 1,2,3; Yuan, Jinghe 4; Lv, Zhizhen 1,2,3; Fang, Xiaohong 4; Li, Zijian 1,2,3; Zhang, Youyi 1,2,3
刊名	FASEB JOURNAL
	2015-06-01
卷号	29 期号:6 页码:2495-2503
关键词	membrane trafficking adaptor protein quantitative live-cell imaging
英文摘要	Actin and dynamin work cooperatively to drive the invagination and scission of clathrin-coated pits (CCPs). However, little is known about the mechanism that orchestrates the spatiotemporal recruitment of dynamin and actin. Here, we have identified the mammalian actin-binding protein 1 (mAbp1; also called HIP-55 or SH3P7), which could bind toclathrin, actin, as well as dynamin, as an adaptor that links the dynamic recruitment of dynamin and actin for the scission of CCPs. Live-cell imaging reveals that mAbp1 is specifically recruited at a late stage of the long-lived CCPs. mAbp1 knockdown impaired CCP scission by reducing dynamin recruitment at the plasma membrane. However, actin disruption remarkably eliminates mAbp1 recruitment and thus dynamin recruitment. These data suggest that by binding to both clathrin and F-actin, mAbp1 is specifically recruited at a late stage of CCP formation, which subsequently recruits dynamin to CCPs.
收录类别	SCI
语种	英语
公开日期	2015-11-02
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28167]
专题	化学研究所_分子纳米结构与纳米技术实验室
作者单位	1.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Key Lab Cardiovascular Mol Biol & Regulatory Pept, Key Lab Mol Cardiovasc Sci,Minist Educ,Minist Hlt, Beijing 100871, Peoples R China 3.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100871, Peoples R China 4.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 5.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	He, Kangmin,Xing, Rui,Yan, Xiaohua,et al. Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction[J]. FASEB JOURNAL,2015,29(6):2495-2503.
APA	He, Kangmin.,Xing, Rui.,Yan, Xiaohua.,Tian, Aiju.,Zhang, Mingliang.,...&Zhang, Youyi.(2015).Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction.FASEB JOURNAL,29(6),2495-2503.
MLA	He, Kangmin,et al."Mammalian actin-binding protein 1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction".FASEB JOURNAL 29.6(2015):2495-2503.