Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish

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	Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish
	Pang, Shao-Chen; Wang, Hou-Peng; Zhu, Zuo-Yan; Sun, Yong-Hua
刊名	MARINE BIOTECHNOLOGY
	2015-10-01
卷号	17 期号:5 页码:593-603
关键词	Gal4/UAS Transcriptional activity Transcriptional silencing DNA methylation Zebrafish
ISSN号	1436-2228
英文摘要	The Gal4/upstream activating sequence (UAS) system is a powerful genetic tool for the temporal and spatial expression of target genes. In this study, the dynamic activity of the Gal4/UAS system was monitored in zebrafish throughout the entire lifespan and during germline transmission, using an optimized Gal4/UAS, KalTA4/4xUAS, which is driven by two muscle-specific regulatory sequences. We found that UAS-linked gene expression was transcriptionally amplified by Gal4/UAS during early developmental stages and that the amplification effects tended to weaken during later stages and even disappear in subsequent generations. In the F2 generation, the transcription of a UAS-linked enhanced green fluorescent protein (EGFP) reporter was transcriptionally silent from 16 days post-fertilization (dpf) into adulthood, yet offspring of this generation showed reactivation of the EGFP reporter in some strains. We further show that the transcriptional silencing and reactivation of UAS-driven EGFP correlated with the DNA methylation levels of the UAS regulatory sequences. Notably, asymmetric DNA methylation of the 4xUAS occurred in oocytes and sperm. Moreover, the paternal and maternal 4xUAS sequences underwent different DNA methylation dynamics after fertilization. Our study suggests that the Gal4/UAS system may represent a powerful tool for tracing the DNA methylation dynamics of paternal and maternal loci during zebrafish development and that UAS-specific DNA methylation should be seriously considered when the Gal4/UAS system is applied in zebrafish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	PRIMORDIAL GERM-CELLS ; GENE-EXPRESSION ; SKELETAL-MUSCLE ; EMBRYO ; GENOME ; PCR
收录类别	SCI
语种	英语
WOS记录号	WOS:000359948100005
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27146]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Pang, Shao-Chen,Wang, Hou-Peng,Zhu, Zuo-Yan,et al. Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish[J]. MARINE BIOTECHNOLOGY,2015,17(5):593-603.
APA	Pang, Shao-Chen,Wang, Hou-Peng,Zhu, Zuo-Yan,&Sun, Yong-Hua.(2015).Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish.MARINE BIOTECHNOLOGY,17(5),593-603.
MLA	Pang, Shao-Chen,et al."Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish".MARINE BIOTECHNOLOGY 17.5(2015):593-603.