Genotypic responses of bacterial community structure to a mixture of wastewater-borne PAHs and PBDEs in constructed mangrove microcosms

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	Genotypic responses of bacterial community structure to a mixture of wastewater-borne PAHs and PBDEs in constructed mangrove microcosms
	Wang, Yafen 1,3,4; Wu, Yan 1; Wu, Zhenbin 3; Tam, Nora Fung-Yee 1,2
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2015-11-15
卷号	298 页码:91-101
关键词	Mangrove constructed wetland PAHs and PBDEs Tidal regime Bacterial community analysis DGGE CCA
ISSN号	0304-3894
英文摘要	Mangrove microcosms capable of removing polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenyl ethers (PBDEs) from wastewater were established under everyday tidal and non-tidal flooding regimes, along with two different mangrove species. Defining how bacterial communities change with pollutants or across treatments will contribute to understanding the microbial ecology of in situ bioremediation systems. A semi-nested PCR-DGGE (denaturing gradient gel electrophoresis) approach was employed, with known genus/species-specific primers targeting the 16S rRNA genes of Sphingomonas and Mycobacterium (related to PAH degradation) and Dehalococcoides (related to PBDE degradation). Results showed that the composition of Mycobacterium- and Dehalococcoides-like populations was critically determined by tidal regime during a medium-term (4-8 months) exposure, while that of Sphingomonas-like population, along with total bacterial community, was more dependent on sediment layer and became prominently affected by tidal regime till the end of 8-month treatment. The effect of plant species was relatively small. Canonical correspondence analysis (CCA) further revealed that Sphingomonas- and Mycobacterium-like populations were significantly associated with phenanthrene and benzo(a)pyrene, respectively, while Dehalococcoides-like population was the only group significantly related to the highest PBDE congener (BDE-209) in the mangrove microcosms. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Technology ; Life Sciences & Biomedicine
类目[WOS]	Engineering, Environmental ; Engineering, Civil ; Environmental Sciences
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
关键词[WOS]	POLYCYCLIC AROMATIC-HYDROCARBONS ; POLYBROMINATED DIPHENYL ETHERS ; COMPLEX MICROBIAL COMMUNITIES ; GEL-ELECTROPHORESIS ANALYSIS ; 16S RIBOSOMAL-RNA ; REDUCTIVE DEBROMINATION ; MYCOBACTERIUM ; DIVERSITY ; BIODEGRADATION ; DEGRADATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000360595800010
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27168]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China 2.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 4.China Univ Geosci, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yafen,Wu, Yan,Wu, Zhenbin,et al. Genotypic responses of bacterial community structure to a mixture of wastewater-borne PAHs and PBDEs in constructed mangrove microcosms[J]. JOURNAL OF HAZARDOUS MATERIALS,2015,298:91-101.
APA	Wang, Yafen,Wu, Yan,Wu, Zhenbin,&Tam, Nora Fung-Yee.(2015).Genotypic responses of bacterial community structure to a mixture of wastewater-borne PAHs and PBDEs in constructed mangrove microcosms.JOURNAL OF HAZARDOUS MATERIALS,298,91-101.
MLA	Wang, Yafen,et al."Genotypic responses of bacterial community structure to a mixture of wastewater-borne PAHs and PBDEs in constructed mangrove microcosms".JOURNAL OF HAZARDOUS MATERIALS 298(2015):91-101.