Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes
	Yang, Ming-kun 1; Yang, Yao-hua 1; Chen, Zhuo 1; Zhang, Jia 1; Lin, Yan 1; Wang, Yan 1; Xiong, Qian 1; Li, Tao 1; Ge, Feng 1; Bryant, Donald A.2
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2014-12-30
卷号	111 期号:52 页码:E5633-E5642
关键词	proteogenomics post-translational modifications cyanobacteria photosynthesis Synechococcus
ISSN号	0027-8424
通讯作者	Li, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
英文摘要	We describe an integrated workflow for proteogenomic analysis and global profiling of posttranslational modifications (PTMs) in prokaryotes and use the model cyanobacterium Synechococcus sp. PCC 7002 (hereafter Synechococcus 7002) as a test case. We found more than 20 different kinds of PTMs, and a holistic view of PTM events in this organism grown under different conditions was obtained without specific enrichment strategies. Among 3,186 predicted protein-coding genes, 2,938 gene products (> 92%) were identified. We also identified 118 previously unidentified proteins and corrected 38 predicted gene-coding regions in the Synechococcus 7002 genome. This systematic analysis not only provides comprehensive information on protein profiles and the diversity of PTMs in Synechococcus 7002 but also provides some insights into photosynthetic pathways in cyanobacteria. The entire proteogenomics pipeline is applicable to any sequenced prokaryotic organism, and we suggest that it should become a standard part of genome annotation projects.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	STRAIN PCC 7002 ; MASS-SPECTROMETRY ; SIGNAL PEPTIDES ; PROTEOME ANALYSIS ; ANALYSIS REVEALS ; CYANOBACTERIA ; IDENTIFICATION ; TRANSCRIPTOME ; ANNOTATION ; GENOME
收录类别	SCI
语种	英语
WOS记录号	WOS:000347444400008
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/23992]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
推荐引用方式 GB/T 7714	Yang, Ming-kun,Yang, Yao-hua,Chen, Zhuo,et al. Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014,111(52):E5633-E5642.
APA	Yang, Ming-kun.,Yang, Yao-hua.,Chen, Zhuo.,Zhang, Jia.,Lin, Yan.,...&Zhao, Jin-dong.(2014).Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,111(52),E5633-E5642.
MLA	Yang, Ming-kun,et al."Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.52(2014):E5633-E5642.