Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody
	Song, Maoyong ; Lv, Xuefei ; Wang, Hailin ; Jiang, Guibin
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2008-04
卷号	390 期号:8 页码:2151-2157
关键词	vitellogenin (Vtg) affinity purification immobilized antibody
英文摘要	Vitellogenin (Vtg) is a highly responsive biomarker for environmental exposure to various estrogenically active compounds. Here we present a simple, fast, mild, and stable immobilization of anti-Vtg antibody, and demonstrate its powerful applications for preconcentration and purification of fish Vtg proteins, allowing for the monitoring and screening of environmental exposure to estrogenically active compounds. In this immobilization method, rabbit antiserum containing a specific polyclonal antibody against Vtg was directly immobilized on an antibody-binding Staphylococcal protein A matrix (SpA) without the need for prior purification. Under the unique elution conditions, the Vtg protein can be eluted out alone without any leaked specific antibody. The developed method was further used to purify Vtg from whole-body homogenate of Chinese rare minnow. Compared with previous purification methods, the isolated Vtg fraction by this method displays higher purity and well-preserved structure integrity. Moreover, our method is eight times faster. The simple one-step protein A-based specific antibody immobilization and its associated elution strategy may be extended to a number of antibodies for various application purposes, highlighting the paramount advantages over traditional immunoprecipitation and covalent immobilization of antibodies, and suggesting a wide range of promising applications in environmental monitoring and proteome analysis.
WOS记录号	WOS:000254755700021
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21948]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Song, Maoyong,Lv, Xuefei,Wang, Hailin,et al. Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2008,390(8):2151-2157.
APA	Song, Maoyong,Lv, Xuefei,Wang, Hailin,&Jiang, Guibin.(2008).Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,390(8),2151-2157.
MLA	Song, Maoyong,et al."Fast purification of trace vitellogenin from Chinese rare minnow using protein A-immobilized antibody".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 390.8(2008):2151-2157.