Simultaneous Determination of Ten Estrogens and their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Simultaneous Determination of Ten Estrogens and their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS
	Yan, Wei ; Zhao, Lixia ; Feng, Qinzhong ; Wei, Yanlin ; Lin, Jin-Ming
刊名	CHROMATOGRAPHIA
	2009-04
卷号	69 期号:1 页码:621-628
关键词	Column liquid chromatography-mass spectrometry Solid phase extraction Water samples analysis Estrogen
中文摘要	Ten highly potent estrogens including estrone (E(1)), 17 beta-estradiol (E(2)), estriol (E(3)), 4-hydroxyestrone (4-OHE(1)), 2-hydroxyestradiol (2-OHE(2)), 16 alpha-hydroxyestrone (16 alpha-OHE(1)), 2-methoxyestrone (2-MeO-E(1)), 2-methoxyestradiol (2-MeO-E(2)), diethylstilbestrol (DES), 17 alpha-ethynylestradiol (EE(2)) were identified and quantified by solid-phase extraction followed by liquid chromatography-mass spectrometry. An improved two-step SPE process was employed in this work. C18 cartridge was used for both enrichment of all target estrogens and retention of some nonpolar impurities, and then a polar florisil cartridge was subsequently used to separate the interested estrogens from the polar impurities. After this pretreatment for water samples, the results showed clean chromatograms without interference from matrix effects. Besides, this method was accurate (recovery percentages between 70.4 and 106.8% for river water and 73.4 and 101.3% for raw sewage, except 4-OHE(1) and 2-OHE(2)), and precise (RSD varying between 1.3 and 17.8% for river water and 3.4 and 16.7% for raw sewage). In the optimized condition, this method was used to verify the presence of the target analytes in the Qinghe River and influent of sewage treatment plant in the northwest of Beijing, China. Some estrogens were detected in the river water and sewage water samples at a relatively high concentration. The developed method proved to be effective for analyzing estrogen compounds in complex matrices. Moreover, the achieved results demonstrated that a great concern should be addressed to the potential risk of the presence of estrogens in the aquatic environment.
WOS记录号	WOS:000264885400005
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/21566]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yan, Wei,Zhao, Lixia,Feng, Qinzhong,et al. Simultaneous Determination of Ten Estrogens and their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS[J]. CHROMATOGRAPHIA,2009,69(1):621-628.
APA	Yan, Wei,Zhao, Lixia,Feng, Qinzhong,Wei, Yanlin,&Lin, Jin-Ming.(2009).Simultaneous Determination of Ten Estrogens and their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS.CHROMATOGRAPHIA,69(1),621-628.
MLA	Yan, Wei,et al."Simultaneous Determination of Ten Estrogens and their Metabolites in Waters by Improved Two-Step SPE Followed by LC-MS".CHROMATOGRAPHIA 69.1(2009):621-628.