NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: Roles of PKC, PKA, ERK, p38 MAPK, and proteasome

	NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: Roles of PKC, PKA, ERK, p38 MAPK, and proteasome
	Zhang, JS; Feng, WG; Li, CL; Wang, XY; Chang, ZL
刊名	CELLULAR IMMUNOLOGY
	2000
卷号	204 期号:1 页码:38-45
关键词	signal transduction transcription factors cytokines
通讯作者	Chang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Lab Immune Signal Transduct, Shanghai 200031, Peoples R China.,
英文摘要	NF-kappa B plays a critical role in coordinating the control of gene expression during monocyte/macrophage activation. In this report we describe our investigation of the mechanisms of LPS-induced NF-kappa B activation and IL-12 expression in murine peritoneal suppressor macrophages. Treatment of these macrophages with LPS induced I kappa B alpha degradation and NF-kappa B activation. EMSAs demonstrated that NF-kappa B bound to a cis-acting element located in the murine IL-12 p40 promoter. LPS signal transduction has been shown to involve a variety of signal pathways. The results in this paper indicate that LPS-induced NF-kappa B binding activity was independent of PKC, PHA, ERK, and p38 MAPK, but was regulated by proteasome. Furthermore, Proteasome Inhibitor I abolished the LPS-induced mRNA expression of IL-12 p35 and p40, and SB203580 reduced these mRNA levels, whereas the blockade of PKC, PKA, and ERK had little effect. These data demonstrate that the LPS-induced activation of proteasome I kappa B NF-kappa B and p38 MAPK signal pathways regulate the IL-12 expression in murine peritoneal suppressor macrophages. (C) 2000 Academic Press.
学科主题	Cell Biology; Immunology
类目[WOS]	Cell Biology ; Immunology
关键词[WOS]	ACTIVATED PROTEIN-KINASE ; TRANSCRIPTION FACTORS ; SIGNAL-TRANSDUCTION ; GENE-EXPRESSION ; REL PROTEINS ; CELLS ; LIPOPOLYSACCHARIDE ; SUBUNIT ; COMPLEX ; PHOSPHORYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000089803200005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2738]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, JS,Feng, WG,Li, CL,et al. NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: Roles of PKC, PKA, ERK, p38 MAPK, and proteasome[J]. CELLULAR IMMUNOLOGY,2000,204(1):38-45.
APA	Zhang, JS,Feng, WG,Li, CL,Wang, XY,&Chang, ZL.(2000).NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: Roles of PKC, PKA, ERK, p38 MAPK, and proteasome.CELLULAR IMMUNOLOGY,204(1),38-45.
MLA	Zhang, JS,et al."NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: Roles of PKC, PKA, ERK, p38 MAPK, and proteasome".CELLULAR IMMUNOLOGY 204.1(2000):38-45.