Primate epididymis-specific proteins: Characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human

	Primate epididymis-specific proteins: Characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human
	Liu, Q; Hamil, KG; Sivashanmugam, P; Grossman, G; Soundararajan, R; Rao, AJ; Richardson, RT; Zhang, YL; O'Rand, MG; Petrusz, P
刊名	ENDOCRINOLOGY
	2001
卷号	142 期号:10 页码:4529-4539
通讯作者	Hall, SH (reprint author), Univ N Carolina, Sch Med, Reprod Biol Lab, CB 7500, Chapel Hill, NC 27599 USA.,
英文摘要	Epididymal secreted proteins promote sperm maturation and fertilizing capacity by interacting with sperm during passage through the epididymis. Here we investigate the molecular basis of sperm maturation by isolating cDNA clones for novel epididymis-specific expressed sequences. Thirty-six novel cDNAs were isolated and sequenced from a subtracted Macaca mulatta epididymis library. The clones encode proteins with a range of motifs characteristic of protein-modifying enzymes, protease inhibitors, hydrophobic ligand-binding and transport proteins, extracellular matrix-interacting proteins, and transcription regulatory factors. The fall length coding sequences were obtained for 11 clones representing a range of abundance levels. Expression of each is regionally localized and androgen regulated. The most abundant, ESC42, contains a cysteine-rich region similar to the signature binding domain of the trefoil family of motogenic wound repair proteins. The monkey and human proteins are nearly 90% identical. Immunohistochemical staining revealed that the protein is most abundant in the epithelium of the caput and is also present in the lumen and bound to sperm. The ESC42 gene, located on chromosome 20q11, contains two exons encoding two nearly identical predicted signal peptides and a third exon encoding the rest of the protein.
学科主题	Endocrinology & Metabolism
类目[WOS]	Endocrinology & Metabolism
关键词[WOS]	PROSTATE-CANCER ; GENE-EXPRESSION ; IDENTIFICATION ; CLONING ; FAMILY ; BINDING ; ACID ; AMPLIFICATION ; RECEPTOR ; PEPTIDES
收录类别	SCI
语种	英语
WOS记录号	WOS:000171238700046
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2705]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, Q,Hamil, KG,Sivashanmugam, P,et al. Primate epididymis-specific proteins: Characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human[J]. ENDOCRINOLOGY,2001,142(10):4529-4539.
APA	Liu, Q.,Hamil, KG.,Sivashanmugam, P.,Grossman, G.,Soundararajan, R.,...&Hall, SH.(2001).Primate epididymis-specific proteins: Characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human.ENDOCRINOLOGY,142(10),4529-4539.
MLA	Liu, Q,et al."Primate epididymis-specific proteins: Characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human".ENDOCRINOLOGY 142.10(2001):4529-4539.