Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene

	Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene
	Zhang, CK; Lin, W; Cai, YN; Xu, PL; Dong, H; Li, M; Kong, YY; Fu, G; Xie, YH; Huang, GM
刊名	GENE
	2001
卷号	273 期号:2 页码:239-249
关键词	nuclear receptor fushi tarazu factor 1 hepatocyte nuclear factors
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	The human homologue of the Drosophila melanogaster orphan nuclear receptor fushi tarazu factor 1 (Ftz-F1),NR5A2(hB1F), was initially identified as a regulatory factor that binds and activates enhancer II of hepatitis B virus. NR5A2 (hB1F) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. A detailed analysis on the genomic structure and promoter activity will greatly promote future studies on the function of the NR5A2 (hB1F) gene. In this report, a bacterial artificial chromosome clone and several phage clones covering the NR5A2 (hB1F) Gene were isolated and the complete genomic sequence was obtained. Alignment of different cDNAs of the NR5A2 (hB1F) gene with the genomic sequence facilitated the delineation of its structural organization, which spans over 150 kb and consists of ei-ht exons interrupted by seven introns. RT-PCR and 3 ' -RACE revealed that utilization of two polyadenylation signals results in the 3.8 and 5.2 kb transcripts that were observed previously. The transcription start site of the NR5A2 (hB1F) gene was mapped downstream of a canonical TATA box. An upstream fragment containing binding sites for several liver-specific and ubiquitous transcription factors exhibits hepatocyte-specific promoter activity. Transient transfections indicated that hepatocyte nuclear factors HNF1 and HNF3 beta could activate NR5A2 (hB1F) promoter. (C) 2001 Elsevier Science B.V. All rights reserved.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	HOMEODOMAIN PROTEIN FTZ ; TRANSCRIPTION FACTOR ; FORK-HEAD ; SUPERFAMILY ; ACTIVATION ; COFACTOR ; SEQUENCE ; SEARCH ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000171151100010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2595]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, CK,Lin, W,Cai, YN,et al. Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene[J]. GENE,2001,273(2):239-249.
APA	Zhang, CK.,Lin, W.,Cai, YN.,Xu, PL.,Dong, H.,...&Wang, Y.(2001).Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene.GENE,273(2),239-249.
MLA	Zhang, CK,et al."Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene".GENE 273.2(2001):239-249.