Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination

	Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination
	Zhao, Y; Chen, X; Peng, WP; Dong, L; Huang, JT; Lu, CD
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2001
卷号	33 期号:1 页码:112-116
关键词	silkworm (Bombyx mori) GFP synthetic fibroin-like gene transgenic homologous recombination
通讯作者	,
英文摘要	A gene unit, which encoded fibroin-like peptide, was synthesized and constructed. The unit was multimerized to about 2 400 bp using BamHI and BglII at each end of the unit, then was fused with gfp reporter gene. The fusion gene, flanked by the 5' and 3' sequence of the fibroin heavy chain gene of silkworm Bombyx: mori, was transferred into the eggs of silkworm by electroporation. After the silkworms developed and spinned silk, 73 out of about 5 400 cocoons were brighter than normal ones under UV light. The protein extracted from the brighter cocoon could react with the GFP polyclonal antibodies. Genomic DNA from these silkworms and their progenies were analyzed. The integration of gfp gene into genomic DNA of silkworm and the occurrence of expected homologous recombination event had been proved by Southern hybridization. It was shown that gfp-fibroin like fusion gene had integrated into the genomic DNA of silkworm by homologous recombination and the phenotype of "brighter cocoon" could be used to select transgenic silkworms.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	SPIDER DRAGLINE SILK ; TRANSGENIC SILKWORM
收录类别	SCI
语种	中文
WOS记录号	WOS:000166598200020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2587]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, Y,Chen, X,Peng, WP,et al. Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):112-116.
APA	Zhao, Y,Chen, X,Peng, WP,Dong, L,Huang, JT,&Lu, CD.(2001).Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),112-116.
MLA	Zhao, Y,et al."Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):112-116.