Isolation of acetylcholinesterase from apoptotic human lung fibroblast cells by antibody affinity chromatography

	Isolation of acetylcholinesterase from apoptotic human lung fibroblast cells by antibody affinity chromatography
	Jin, QH; Shi, YF; He, HY; Ng, KKW; Jiang, H; Yang, L; Jiang, ZQ; Zhang, XJ
刊名	BIOTECHNIQUES
	2002
期号	1 页码:92-+
通讯作者	Zhang, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 YueYang Rd, Shanghai 200031, Peoples R China.,
英文摘要	Acetylcholinesterase (AChE; EC3.1.1.7) is well known for its role in the hydrolysis of acetylcholine at cholinergic synapses to terminate neurotransmission. In addition to its synaptic presence, AChE has been found to be in non-cholinergic cells such as hematopoietic and osteogenic cells. We have recently reported that AChE is expressed in various cells undergoing apoptosis. To characterize AChE in apoptotic cells and to investigate the role of AChE expression in apoptosis, we devised a method to purify AChE expressed in apoptotic human lung fibroblast cell line HLF. The isolation of this enzyme is mainly based on inhibitor ligand affinity chromatography using immobilized tacrine. However, this method is only effective in isolating active AChE Here we employed antibody-based chromatography and found that both active and inactive AChE were present in apoptotic HLF cells. Active AChE was predominantly observed in the nuclei of apoptotic cells, while inactive AChE was mainly present in the cytoplasm. Therefore, our method provides an opportunity to investigate further the role of AChE especially inactive AChE, in apoptosis.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
关键词[WOS]	TISSUE-SPECIFIC EXPRESSION ; IN-SITU HYBRIDIZATION ; MOLECULAR-FORMS ; MESSENGER-RNA ; MAMMALIAN ACETYLCHOLINESTERASE ; ALZHEIMERS-DISEASE ; CHICKEN BRAIN ; RAT-BRAIN ; GENE ; CHOLINESTERASES
收录类别	SCI
语种	英语
WOS记录号	WOS:000178765500011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2567]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jin, QH,Shi, YF,He, HY,et al. Isolation of acetylcholinesterase from apoptotic human lung fibroblast cells by antibody affinity chromatography[J]. BIOTECHNIQUES,2002(1):92-+.
APA	Jin, QH.,Shi, YF.,He, HY.,Ng, KKW.,Jiang, H.,...&Zhang, XJ.(2002).Isolation of acetylcholinesterase from apoptotic human lung fibroblast cells by antibody affinity chromatography.BIOTECHNIQUES(1),92-+.
MLA	Jin, QH,et al."Isolation of acetylcholinesterase from apoptotic human lung fibroblast cells by antibody affinity chromatography".BIOTECHNIQUES .1(2002):92-+.