Self-aggregation of the structural protein encoded by rice ragged stunt oryzavirus genome segment 8

	Self-aggregation of the structural protein encoded by rice ragged stunt oryzavirus genome segment 8
	Lu, HJ; Shao, CG; Gong, ZX
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2002
卷号	34 期号:5 页码:565-570
关键词	RRSV P8 structural protein self-cleavage self-aggregation
通讯作者	,
英文摘要	Rice ragged stunt oryzavirus (RRSV) is a member of the genus oryzavirus within the family Reoviridae. Its genome consists of ten segments of dsRNA. The functions of all products encoded by these viral genome segments, except one encoded by S9, have not yet been elucidated. In the present sturdy, the ORF of S8 of RRSV-Philippines isolate was sequenced and expressed in E. cold. The 67 kD product of S8 could be self-cleaved into two fragments with molecular weights of 43 kD and 26 kD. Western blotting indicated that both 67 kD and 43 kD products were major structural proteins of the virus. It was also found that the 67 kD protein could self-aggregate into aggregates with higher sedimentation rate in sucrose gradients during centrifugation. Moreover, the self-aggregation process could be accelerated by the complex of S6 product and genome dsRNAs of RRSV. These results suggest that the S8 products, 67 kD or 43 kD, may be, the structural components of the viral inner-capsid.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	TRANSMISSION
收录类别	SCI
语种	中文
WOS记录号	WOS:000178180200006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2503]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lu, HJ,Shao, CG,Gong, ZX. Self-aggregation of the structural protein encoded by rice ragged stunt oryzavirus genome segment 8[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(5):565-570.
APA	Lu, HJ,Shao, CG,&Gong, ZX.(2002).Self-aggregation of the structural protein encoded by rice ragged stunt oryzavirus genome segment 8.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(5),565-570.
MLA	Lu, HJ,et al."Self-aggregation of the structural protein encoded by rice ragged stunt oryzavirus genome segment 8".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.5(2002):565-570.