The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast

	The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast
	Chen, Y; Zhao, MJ; Li, ZP; He, ML
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	299 期号:5 页码:723-729
关键词	Attacus ricini rDNA matrix attachment region autonomously replicating sequenced replication origin yeast
通讯作者	He, ML (reprint author), Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China.,
英文摘要	Nuclear matrix attachment regions (MARs) play a crucial role in chromatin architecture, gene expression, and DNA replication. Although it is well known that yeast autonomously replicating sequences (ARSs) bind nuclear matrix and MARs also function as ARS elements in yeast, whether a heterologous MAR or ARS element acts as a replication origin in the chromosome has not been elucidated. We previously identified a MAR (rMAR) located in the nontranscribed spacer (NTS) of silkworm Attacus ricini rDNA. We report here that this rMAR contains 10 copies of ARS consensus sequence (ACS) and several DNA unwinding regions. The rMAR employs ARS activity in yeast and a rARS element locates in the 3' region of the rMAR. Furthermore, we have also revealed that either the rMAR or the rARS element functions as a replication origin in the chromosome. Our results provide the first direct evidence to demonstrate that heterologous rMAR and rARS display chromosomal origin activity, suggesting that the chromosome structure and replication origin of rDNA reserve some common features during evolution. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RIBOSOMAL-RNA GENES ; DNA-REPLICATION ; SACCHAROMYCES-CEREVISIAE ; BUDDING YEAST ; FORK BARRIERS ; ELEMENTS ; SCAFFOLD ; DYNAMICS ; TRANSCRIPTION ; INITIATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000180886900008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2495]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, Y,Zhao, MJ,Li, ZP,et al. The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,299(5):723-729.
APA	Chen, Y,Zhao, MJ,Li, ZP,&He, ML.(2002).The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,299(5),723-729.
MLA	Chen, Y,et al."The function of the nuclear matrix attachment region of silkworm rDNA as an autonomously replicating sequence in plasmid and chromosomal replication origin in yeast".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 299.5(2002):723-729.