Analysis of phosphorylation of YJL084c, a yeast protein

	Analysis of phosphorylation of YJL084c, a yeast protein
	Shi, XZ; Ao, SZ
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2002
卷号	34 期号:4 页码:433-438
关键词	PCL6 PCL7 YJL084c phosphorylation yeast two-hybrid system
通讯作者	,
英文摘要	PCL6, PCL7(PAP1), and PHO80 belong to the PHO80 subfamily of PCLs (PHO85 cyclins), share high homology in protein sequences, and function with some similarity. YLR190w, the substrate of PCL7-PHO85, shares homology with YJL084c in a 140-amino-acid region. In addition, YJL084c was reported as a PCL6-binding protein. Here, it was found that there was association between YJL084c and PCL7, and their interaction was confirmed by co-immunoprecipitation assay and GST pull-down assay. The in vitro translational product of YJL084c could be phosphorylated by PCL7-PHO85 complex. Also, the GST fusion protein of the middle region expressed in E. coli could be phosphorylated, while the amino terminal or the carboxyl terminal could not. Interestingly, PCL6-PHO85 complex had the same characters; effect of phosphate condition on the phosphorylation was shown in both PCL6-PHO85 and PCL7-PHO85. YPH499: Yjl084c :: LEU2 was constructed by homologous recombination. PUT4 was reported as a YJL084c-binding protein, but no difference was observed between wild strain and the Yjl084c null mutant on MP medium. In addition, the interaction between PHO81 and all of the three cyclins was analyzed.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CYCLIN-DEPENDENT KINASE ; SACCHAROMYCES-CEREVISIAE ; INHIBITOR PHO81 ; CDK COMPLEX ; EXPRESSION ; FAMILY ; GENE ; PHO80-PHO85 ; CLONING
收录类别	SCI
语种	中文
WOS记录号	WOS:000177017300008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2481]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Shi, XZ,Ao, SZ. Analysis of phosphorylation of YJL084c, a yeast protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):433-438.
APA	Shi, XZ,&Ao, SZ.(2002).Analysis of phosphorylation of YJL084c, a yeast protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),433-438.
MLA	Shi, XZ,et al."Analysis of phosphorylation of YJL084c, a yeast protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):433-438.