Expression and functional analysis of an inhibitor of apoptosis protein from Trichoplusia ni

	Expression and functional analysis of an inhibitor of apoptosis protein from Trichoplusia ni
	Liao, WT; Yang, Y; Wu, XF
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2002
卷号	293 期号:2 页码:675-679
关键词	apoptosis inhibitor of apoptosis protein caspase expression
通讯作者	Wu, XF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要	An inhibitor of the apoptosis protein (IAP) family gene from Trichoplusia ni, Tn-IAP1v, a variant of lepidopteran Tn-IAP1, was cloned by RT-PCR. There are six single nucleotide polymorphisms between the two Tn-IAP1 variants, resulting in three predicted single amino acid polymorphisms. With the GST fusion expression system, soluble recombinant Tn-IAP1v was highly expressed in Escherichia coli and then purified by affinity chromatography. Caspase inhibition assays indicated that recombinant Tn-IAP1v could specifically inhibit human caspase-9 in vitro instead of caspase-3, -7, and -8, which was further confirmed by the observation that recombinant Tn-IAP1v can directly bind caspase-9 in the protein pull-down assay. These results suggested that Tn-IAP1v might serve as an initiator caspase inhibitor in vivo in the conserved mitochondria apoptotic pathway. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CYTOCHROME-C ; CELL-DEATH ; IAP ; BACULOVIRUS ; COMPLEX
收录类别	SCI
语种	英语
WOS记录号	WOS:000175488600003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2438]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liao, WT,Yang, Y,Wu, XF. Expression and functional analysis of an inhibitor of apoptosis protein from Trichoplusia ni[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,293(2):675-679.
APA	Liao, WT,Yang, Y,&Wu, XF.(2002).Expression and functional analysis of an inhibitor of apoptosis protein from Trichoplusia ni.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,293(2),675-679.
MLA	Liao, WT,et al."Expression and functional analysis of an inhibitor of apoptosis protein from Trichoplusia ni".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 293.2(2002):675-679.