Identification of a NF-kappa B site in the negative regulatory element (epsilon-NRAII) of human epsilon-globin gene and its binding protein NF-kappa B p50 in the nuclei of K562 cells

	Identification of a NF-kappa B site in the negative regulatory element (epsilon-NRAII) of human epsilon-globin gene and its binding protein NF-kappa B p50 in the nuclei of K562 cells
	Hou, CH; Huang, J; Qian, RL
刊名	CELL RESEARCH
	2002
卷号	12 期号:1 页码:79-82
关键词	human epsilon-globin gene negative regulatory element NF-kappa B p50
通讯作者	Qian, RL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Grp Globin Gene Express &, Shanghai 200031, Peoples R China.,
英文摘要	The developmental control of the human epsilon-globin gene expression is mediated by transcription regulatory elements in the 5' flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-kappaB consensus sequence resides in the negative regulatory element (-3028bp similar to -2902bp, termed epsilon-NRAII) 5' to the cap site of this gene. NRF DNA fragment (-3010bp similar to -2986bp) containing the NF-kappaB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-kappaB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-kappaB motif) could not, suggesting that the binding protein is a member of NF-kappaB/Rel family. Western blot assay demonstrated that the molecular weight of NF-kappaB protein in the nuclei of K562 cells is 50ku. We suggested that NF-kappaB p50 may play an important role in the regulation of human epsilon-globin gene expression.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	TRANSGENIC MICE ; DEVELOPMENTAL CONTROL ; TRANSCRIPTION ; SPECIFICITY ; EXPRESSION ; SILENCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000174833300010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2420]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hou, CH,Huang, J,Qian, RL. Identification of a NF-kappa B site in the negative regulatory element (epsilon-NRAII) of human epsilon-globin gene and its binding protein NF-kappa B p50 in the nuclei of K562 cells[J]. CELL RESEARCH,2002,12(1):79-82.
APA	Hou, CH,Huang, J,&Qian, RL.(2002).Identification of a NF-kappa B site in the negative regulatory element (epsilon-NRAII) of human epsilon-globin gene and its binding protein NF-kappa B p50 in the nuclei of K562 cells.CELL RESEARCH,12(1),79-82.
MLA	Hou, CH,et al."Identification of a NF-kappa B site in the negative regulatory element (epsilon-NRAII) of human epsilon-globin gene and its binding protein NF-kappa B p50 in the nuclei of K562 cells".CELL RESEARCH 12.1(2002):79-82.