Expression of human alpha-galactosidase leads to reduction of major xenoepitope Gal alpha(1,3) Gal in NIH 3T3 cell

	Expression of human alpha-galactosidase leads to reduction of major xenoepitope Gal alpha(1,3) Gal in NIH 3T3 cell
	Yan, JL; Yu, LY; Zhu, LH; Guo, LH
刊名	ACTA PHARMACOLOGICA SINICA
	2003
卷号	24 期号:10 页码:985-990
关键词	human alpha-galactosidase Gal epitope xenotransplantation NIH 3T3 cell
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,lhguo@sunm.shcnc.ac.cn
英文摘要	AIM: To examine the effects of the expression of alpha-galactosidase on the expression of the major xenoepitope Gal alpha(1,3) Gal (G antigen) in NIH 3T3 cell. METHODS: The expression levels of G antigen and H antigen and binding of human natural antibodies (IgG and IgM) and complement (C3c) to NIH 3T3 cells were analyzed by flow cytometry. Western blot was employed to further determine the expression of glycoproteins of G antigen. Cytolysis assay with normal human serum was performed by MTT assay. RESULTS: In transfectants, Western blot showed that the binding of human IgG to glycosylated proteins located on the cell membrane was decreased, even abrogated totally. Together with the reduced binding of Gs-IB4 (Griffonia simplicifolia) to transfectants, the stable expression of human alpha-galactosidase effectively inhibited Galalpha(1,3) Gal, Gal epitope synthesis in NIH 3T3 cell. As a result, the xenoreactivities of human IgG, IgM, and C3c were reduced by 73.4 %, 22.3 % and 47.9 respectively, while the cell lysis mediated by human XNA and complements was decreased by 42.4 % CONCLUSION: The stable expression of human alpha-galactosidase in NIH 3T3 cell strongly inhibits the expression of Gal epitopes, resulting in abrupt reduction in xenorejection induced by human serum.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	HYPERACUTE REJECTION ; STRUCTURAL ORGANIZATION ; NATURAL ANTIBODIES ; MULTIPLE ORGANS ; TRANSGENIC MICE ; HUMAN SERUM ; FABRY MICE ; GROUP-B ; GROUP-O ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000185749400005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2357]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yan, JL,Yu, LY,Zhu, LH,et al. Expression of human alpha-galactosidase leads to reduction of major xenoepitope Gal alpha(1,3) Gal in NIH 3T3 cell[J]. ACTA PHARMACOLOGICA SINICA,2003,24(10):985-990.
APA	Yan, JL,Yu, LY,Zhu, LH,&Guo, LH.(2003).Expression of human alpha-galactosidase leads to reduction of major xenoepitope Gal alpha(1,3) Gal in NIH 3T3 cell.ACTA PHARMACOLOGICA SINICA,24(10),985-990.
MLA	Yan, JL,et al."Expression of human alpha-galactosidase leads to reduction of major xenoepitope Gal alpha(1,3) Gal in NIH 3T3 cell".ACTA PHARMACOLOGICA SINICA 24.10(2003):985-990.