Characterization of a strong repression domain in the huge region of orphan nuclear receptor hB1F/hLRH-1

	Characterization of a strong repression domain in the huge region of orphan nuclear receptor hB1F/hLRH-1
	Xu, PL; Shan, SF; Kong, YY; Xie, YH; Wang, YA
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003
卷号	35 期号:10 页码:909-916
关键词	hB1F/hLRH-1 orphan nuclear receptor hinge region repression domain HBV enhancer II/core promoter
通讯作者	Xie, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,
英文摘要	Human hepatitis B virus enhancer II B1 binding factor (hB1F,also known as NR5A2, LRH-1, FTF or CPF) is an orphan nuclear receptor and belongs to the fushi tarazu factor I (FTZ-F1) subfamily. It plays important roles in the transcriptional regulation of a number of genes involved in bile acid biosynthesis pathway, hepatitis B virus (HBV) replication and liver specific regulatory network. Like other nuclear receptors, hB1F is composed of modular functional domains. We characterized a domain in its hinge region that imposes a strong repression on the transcriptional activity of hB1F, which is important for the function of hB1F on regulating the activity of HBV enhancer II/core promoter. Mutations of the core residues in this domain abrogate the repression. Bioinformatic analysis reveals that the amino acid sequence of this region is highly conserved only among members of the FTZ-F1 subfamily. The repression is observed in five cell lines tested, while the degree of the repression varies greatly, which does not parallel with the expression level of the DEAD box protein of 130 kD (DP103), a potential interacting protein of a homologous domain in the steroidogenic factor 1 (SF-1). Moreover, the repression is not affected by the silencing mediator for retinoic acid receptor and thyroid hormone receptor (SMRT) and steroid receptor coactivator 1 (SRC-1). Collectively, these data suggest a novel regulatory mechanism for the transcriptional activity of hB1F.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	PROTEIN SECONDARY STRUCTURE ; HEPATITIS-B VIRUS ; TRANSCRIPTION FACTOR ; COACTIVATOR ; EXPRESSION ; HOMOLOG-1 ; GENE ; SUPERFAMILY ; FTZ-F1 ; BOX
收录类别	SCI
语种	英语
WOS记录号	WOS:000186075100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2353]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, PL,Shan, SF,Kong, YY,et al. Characterization of a strong repression domain in the huge region of orphan nuclear receptor hB1F/hLRH-1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(10):909-916.
APA	Xu, PL,Shan, SF,Kong, YY,Xie, YH,&Wang, YA.(2003).Characterization of a strong repression domain in the huge region of orphan nuclear receptor hB1F/hLRH-1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(10),909-916.
MLA	Xu, PL,et al."Characterization of a strong repression domain in the huge region of orphan nuclear receptor hB1F/hLRH-1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.10(2003):909-916.