Involvement of dopamine D3 and neuropeptide YY5 receptors in diabetic gastroparetic rats without response to erythromycin

	Involvement of dopamine D3 and neuropeptide YY5 receptors in diabetic gastroparetic rats without response to erythromycin
	Qin, XY; Wang, ZG; Fei, J; Liu, FL; Cui, DF; Chen, SL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003
卷号	35 期号:9 页码:811-815
关键词	diabetic gastroparesis erythromycin DNA microarray RT-PCR
通讯作者	Qin, XY (reprint author), Fudan Univ, Zhongshan Hosp, Dept Gen Surg, Shanghai 200032, Peoples R China.,
英文摘要	Erythromycin may accelerate gastric emptying in animals and human probably as an motilin agonist, but its prokinetic effects show obvious individual disparity. This study was to find the mechanism of this phenomenon. Microarray analysis was used to screen genes that might be involved in the response of diabetic gastroparesis rats to erythromycin. It was found that erythromycin accelerated gastric emptying of diabetic rats with great individual disparity. Through microarray analysis we screened differential expression genes that might be involved in the effect of erythromycin. Among 10 genes screened out, dopamine D3 receptor (DRD3) and neuropeptide Y Y5 receptor (NPYY5) genes were submitted to RT-PCR quantification and showed consistent results with microarray. It can be concluded that erythromycin promote gastric emptying of gastroparetic rats; DRD3 and NPYY5 may be involved in prokinetic action of erythromycin; and targets other than motilin receptor of erythromycin might exist as prokinetics.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	MOTILIN ; LYMPHOCYTES ; AGONIST ; AGENTS ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000185493600006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2342]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qin, XY,Wang, ZG,Fei, J,et al. Involvement of dopamine D3 and neuropeptide YY5 receptors in diabetic gastroparetic rats without response to erythromycin[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(9):811-815.
APA	Qin, XY,Wang, ZG,Fei, J,Liu, FL,Cui, DF,&Chen, SL.(2003).Involvement of dopamine D3 and neuropeptide YY5 receptors in diabetic gastroparetic rats without response to erythromycin.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(9),811-815.
MLA	Qin, XY,et al."Involvement of dopamine D3 and neuropeptide YY5 receptors in diabetic gastroparetic rats without response to erythromycin".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.9(2003):811-815.