A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity

	A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity
	Zhan, JB; Chen, XY; Wang, CM; Qiu, JW; Ma, FS; Wang, KY; Zheng, S
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2003
卷号	311 期号:2 页码:495-500
关键词	Conus magus conotoxins analgesia ion channels
通讯作者	Zhan, JB (reprint author), Zhejiang Univ, Sch Med, Dept Biochem, 353 Yan An Rd, Hangzhou 310006, Peoples R China.,
英文摘要	omega-Conotoxin MVIIA (CTX MVIIA) is a potent and selective blocker of the N-type voltage-sensitive calcium channel in neurons. Its analgesic and neuroprotective effects may prove useful in treatment of severe pains and ischemia. In this paper, we report that a fusion form of CTX MVIIA with thioredoxin (Trx) has analgesic function. The DNA fragments were chemically synthesized and ligated to form the DNA sequence encoding CTX MVIIA. The synthetic gene was then cloned into the expression vector pET-32a(+) and the fusion protein Trx-CTX MVIIA containing 6x His-tag was purified by one-step metal chelated affinity chromatography (MCAC). The purity of final product was over 95% determined by HPLC and the yield of the fusion protein was approximately 40 mg/L. The analgesic function was detected by using mouse hot-plate assay. After intracranially administering fusion protein with the dose of 0.6 mg/kg, marked analgesia was observed. The analgesic effects (elevated pain thresholds) were dose-dependent and the biological half-life of the fusion toxin was approximately 1.6 h. (C) 2003 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CALCIUM-CHANNEL BLOCKER ; VENOM PEPTIDES ; RATS ; ZICONOTIDE ; ISCHEMIA ; MORPHINE ; SNX-111
收录类别	SCI
语种	英语
WOS记录号	WOS:000186584100039
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2243]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhan, JB,Chen, XY,Wang, CM,et al. A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,311(2):495-500.
APA	Zhan, JB.,Chen, XY.,Wang, CM.,Qiu, JW.,Ma, FS.,...&Zheng, S.(2003).A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,311(2),495-500.
MLA	Zhan, JB,et al."A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 311.2(2003):495-500.