Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma

	Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma
	Liao, C; Zhao, MJ; Zhao, J; Song, H; Pineau, P; Marchio, A; Dejean, A; Tiollais, P; Wang, HY; Li, TP
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2003
卷号	9 期号:1 页码:89-93
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要	AIM: To find the point mutations meaningful for inactivation of liver-related putative tumor suppressor gene (LPTS) gene, a human novel liver-related putative tumor suppressor gene and telomerase inhibitor in hepatocellular carcinoma. METHODS: The entire coding sequence of LPTS gene was examined for mutations by single strand conformation polymorphism (SSCP) assay and PCR products direct sequencing in 56 liver cancer cell lines, 7 ovarian cancer and 7 head & neck tumor cell lines and 70 pairs of HCC tissues samples. The cDNA fragment coding for the most frequent mutant protein was subcloned into GST fusion expression vector. The product was expressed in E.coli and purified by glutathione-agarose column. Telomeric repeat amplification protocol (TRAP) assays were performed to study the effect of point mutation to telomerase inhibitory activity. RESULTS: SSCP gels showed the abnormal shifting bands and DNA sequencing found that there were 5 different mutations and/or polymorphisms in 12 tumor cell lines located at exon2, exon5 and exon7. The main alterations were A(778)A/G and A(880)T in exon7. The change in site of 778 could not be found in HCC tissue samples, while the mutation in position 880 was seen in 7 (10 %) cases. The mutation in the site of 880 had no effect on telomerase inhibitory activity. CONCLUSION: Alterations identified in this study are polymorphisms of LPTS gene. LPTS mutations occur in HCC but are infrequent and of little effect on the telomerase inhibitory function of the protein. Epigenetics, such as methylation, acetylation, may play the key role in inactivation of LPTS.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
关键词[WOS]	LUNG-CANCER ; HETEROZYGOSITY ; IDENTIFICATION ; LOCI
收录类别	SCI
语种	英语
WOS记录号	WOS:000180666400019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2241]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liao, C,Zhao, MJ,Zhao, J,et al. Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(1):89-93.
APA	Liao, C.,Zhao, MJ.,Zhao, J.,Song, H.,Pineau, P.,...&Li, TP.(2003).Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,9(1),89-93.
MLA	Liao, C,et al."Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 9.1(2003):89-93.