Molecular cloning and characterization of rat karyopherin alpha 1 gene: structure and expression

	Molecular cloning and characterization of rat karyopherin alpha 1 gene: structure and expression
	Wang, BW; Li, ZH; Xu, L; Goggi, J; Yu, Y; Zhou, JW
刊名	GENE
	2004
卷号	331 期号:1 页码:149-157
关键词	6-hydroxydopamine brain injury importin alpha karyopherin alpha
通讯作者	Zhou, JW (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Key Lab Proteom, Bldg 23,Room 316,320 Yueyang Rd, Shanghai 200031, Peoples R China.,jwzhou@sibs.ac.cn
英文摘要	Dopamine denervation in the striata of patients with Parkinson's disease (PD) leads to changes in neural plasticity. However, the mechanisms leading to the changes are still poorly understood. In an effort to study the molecular events in the denervated striatum, we identified and cloned rat karyopherin alpha1 (KPNA1), a member of the importin/karyopherin alpha (KPNA) family. DNA sequence analysis revealed that the full-length cDNA, encoding rat KPNA I, was 4975 bp with a short 5'-untranslated region (UTR) of 70 bp, a putative coding sequence of 1617 bp, and an unusually long 3'-UTR of 3266 bp. The gene shared a high degree of similarity with its mouse and human homologs at both cDNA and protein levels. By computational analysis of its genomic sequence, the transcription unit was shown to span a 44-kb region and consist of 13 exons varying in size from 89 (6th exon) to 3454 bp (13th exon), and 12 introns varying in size from 0.3 to 8.9 kb. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that KPNA1 transcript existed in various adult tissues. Both Northern blot and semi-quantitative RT-PCR analysis showed that the expression level of KPNA I mRNA was altered in the denervated striatum post-lesion in a time-dependent manner, reaching the maximum at 2 weeks post-lesion. Our results suggest involvement of KPNA1 in the striatal responses to denervation following 6-hydroxydopamine (6-OHDA)-induced lesion. (C) 2004 Elsevier B.V. All rights reserved.
学科主题	Genetics & Heredity
类目[WOS]	Genetics & Heredity
关键词[WOS]	NUCLEAR TRANSPORT FACTOR ; IMPORTIN-ALPHA ; DIFFERENTIAL EXPRESSION ; PARKINSONS-DISEASE ; STRIATAL EXTRACTS ; PROTEIN IMPORT ; GROWTH ; RNA ; INTERACTS ; CULTURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000221495400016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2207]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, BW,Li, ZH,Xu, L,et al. Molecular cloning and characterization of rat karyopherin alpha 1 gene: structure and expression[J]. GENE,2004,331(1):149-157.
APA	Wang, BW,Li, ZH,Xu, L,Goggi, J,Yu, Y,&Zhou, JW.(2004).Molecular cloning and characterization of rat karyopherin alpha 1 gene: structure and expression.GENE,331(1),149-157.
MLA	Wang, BW,et al."Molecular cloning and characterization of rat karyopherin alpha 1 gene: structure and expression".GENE 331.1(2004):149-157.