A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses

	A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses
	Zhu, LX; Liu, J; Ye, Y; Xie, YH; Kong, YY; Li, GD; Wang, Y
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2004
卷号	10 期号:17 页码:2488-2492
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Yue Yang Rd 320, Shanghai 200031, Peoples R China.,wangy@sibs.ac.cn
英文摘要	AIM: To investigate the immunogenicity of candidate DNA vaccine against hepatitis C virus (HCV) delivered by two plasmids expressing HCV envelope protein 1 (E1) and envelope protein 2 (E2) antigens respectively and to study the effect of CpG adjuvant on this candidate vaccine. METHODS: Recombinant plasmids expressing HCV E1 and E2 antigens respectively were used to simultaneously inoculate mice with or without CpG adjuvant. Antisera were then collected and titers of anti-HCV antibodies were analyzed by ELISA. One month after the last injection, animals were sacrificed to prepare single-cell suspension of splenocytes. These cells were subjected to HCV antigen specific proliferation assays and cytokine secretion assays to evaluate the cellular immune responses of the vaccinated animals. RESULTS: Antibody responses to HCV E1 and E2 antigens were detected in vaccinated animals. Animals receiving CpG adjuvant had slightly lower titers of anti-HCV antibodies in the sera, while the splenocytes from these animals showed higher HCV-antigen specific proliferation. Analysis of cytokine secretion from the splenocytes was consistent with the above results. While no antigen-specific IL-4 secretion was detected for all vaccinated animals, HCV antigen-specific INF-gamma secretion was detected for the splenocytes of vaccinated animals. CpG adjuvant enhanced the secretion of INF-gamma but did not change the profile of IL-4 secretion. CONCLUSION: Vaccination of mice with plasmids encoding HCV E1 and E2 antigens induces humoral and cellular immune responses. CpG adjuvant significantly enhances the cellular immune response.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
收录类别	SCI
语种	英语
WOS记录号	WOS:000208096900006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2169]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhu, LX,Liu, J,Ye, Y,et al. A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(17):2488-2492.
APA	Zhu, LX.,Liu, J.,Ye, Y.,Xie, YH.,Kong, YY.,...&Wang, Y.(2004).A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses.WORLD JOURNAL OF GASTROENTEROLOGY,10(17),2488-2492.
MLA	Zhu, LX,et al."A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses".WORLD JOURNAL OF GASTROENTEROLOGY 10.17(2004):2488-2492.